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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FcERI / Fc Epsilon RI Antibody (aa144‑193) LS‑C111011
Fc Epsilon RI antibody LS-C111011 is an unconjugated rabbit polyclonal antibody to Fc Epsilon RI (FcERI) from human, monkey and rabbit. Validated for WB.
Catalog
Size
Price
LS-C111011-50
50 µg
$365

Popular FcERI / Fc Epsilon RI Products

Anti-FCER1A antibody IHC of human mast cell in human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
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Product Description

Fc Epsilon RI antibody LS-C111011 is an unconjugated rabbit polyclonal antibody to Fc Epsilon RI (FcERI) from human, monkey and rabbit. Validated for WB.

Specifications

Target
Human FcERI / Fc Epsilon RI
Synonyms
FCER1A, Alpha polypeptide, FCE1A, FcERI, Fc Epsilon RI, Fc epsilon RI alpha-chain, Fc-epsilon RI-alpha, IgE Fc receptor subunit alpha
Host
Rabbit
Reactivity
Human, Monkey, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Failed Applications
  • IHC - Paraffin
Immunogen
Synthetic peptide located between aa144-193 of human FCER1A (P12319, NP_001992). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Drosophila (90%); Rabbit (84%).
Blocking Peptide
LS-E16557 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111011. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa144-193
Specificity
Human FCER1A
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About FcERI / Fc Epsilon RI
Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. P12319 NM_002001 NP_001992.1


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Images

Western blot

FCER1A antibody Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FCER1A antibody Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FCER1A antibody Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FCER1A antibody Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FCER1A antibody Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FCER1A antibody Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FCER1A antibody Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FCER1A antibody Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/13/2018

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