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Catalog Number	Size	Price
LS-C662101-10	10 µg	$318
LS-C662101-100	100 µg	$470

FABP4 / AP2 Antibody - Western blot analysis of FABP4 using anti-FABP4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat cardiac muscle tissue lysate,Lane 2: mouse cardiac muscle tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FABP4 at approximately 15KD. The expected band size for FABP4 is at 15KD.

Monoclonal Rabbit anti‑Rat FABP4 / AP2 Antibody (WB) LS‑C662101

Name: Anti-FABP4 / AP2 Antibody | Rabbit anti-Rat Monoclonal ELISA,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662101-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

FABP4 / AP2 Rabbit anti-Rat Monoclonal Antibody

Application:

WB, ELISA

Reactivity:

Rat, Mouse

Format:

Unconjugated, Unmodified

$318

LS-C662101-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

FABP4 / AP2 Rabbit anti-Rat Monoclonal Antibody

Application:

WB, ELISA

Reactivity:

Rat, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

AP2 antibody LS-C662101 is an unconjugated rabbit monoclonal antibody to AP2 (FABP4) from rat. It is reactive with mouse and rat. Validated for ELISA and WB.

Target

Rat FABP4 / AP2

Synonyms

See All FABP4 / AP2 Antibodies

Host

Rabbit

Reactivity

Rat, Mouse (tested or 100% immunogen sequence identity)

Clonality

Monoclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E. coli-derived rat FABP4 recombinant protein (Position: C2-A132). Rat FABP4 shares 88.5% and 93.9% amino acid (aa) sequence identity with human and mouse FABP4, respectively.

Applications

Western blot
ELISA

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About FABP4 / AP2

Uniprot: P15090 NCBI: NM_001442 NP_001433.1