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Antibodies > EZR / Ezrin > LS-C808456
 
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Catalog Number Size Price
LS-C808456-100 100 µl (1 mg/ml) $379 
LS-C808456-200 200 µl (1 mg/ml) $421 
EZR / Ezrin Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
EZR / Ezrin Antibody - Western blot analysis of Phospho-Ezrin (Tyr146) antibody expression in UV treated 293 cells lysates. The lane on the right is treated with the antigen-specific peptide.
EZR / Ezrin Antibody - Western blot analysis of Ezrin phosphorylation expression in UV treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
EZR / Ezrin Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
EZR / Ezrin Antibody - Western blot analysis of Phospho-Ezrin (Tyr146) antibody expression in UV treated 293 cells lysates. The lane on the right is treated with the antigen-specific peptide.
EZR / Ezrin Antibody - Western blot analysis of Ezrin phosphorylation expression in UV treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human EZR / Ezrin Antibody (phospho‑Tyr146, IF, WB) LS‑C808456

Polyclonal Rabbit anti‑Human EZR / Ezrin Antibody (phospho‑Tyr146, IF, WB) LS‑C808456

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Antibody:
EZR / Ezrin Rabbit anti-Human Polyclonal (pTyr146) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C808456-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
EZR / Ezrin Rabbit anti-Human Polyclonal (pTyr146) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Ezrin antibody LS-C808456 is an unconjugated rabbit polyclonal antibody to Ezrin (EZR) (pTyr146) from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
Target
Human EZR / Ezrin
Synonyms
EZR | CVIL | Cytovillin | CVL | p81 | VIL2 | Villin 2 (ezrin) | Villin-2 | Cytovillin 2 | Ezrin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Ezrin around the phosphorylation site of Tyrosine 146.
Epitope
pTyr146
Specificity
Phospho-Ezrin (Tyr146) Antibody detects endogenous levels of Ezrin only when phosphorylated at Tyrosine 146.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EZR / Ezrin
P15311 NM_003379 NP_003370.2

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Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
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Applications: Western blot, ELISA
EZR / Ezrin Antibody - Immunohistochemical analysis of Ezrin (pT567) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
EZR / Ezrin Antibody (phospho‑Thr567) LS‑C352992
Species: Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
EZR / Ezrin Antibody - Western blot analysis of Ezrin expression in HeLa (A); A431 (B); SHSY5Y (C) whole cell lysates.
EZR / Ezrin Antibody (C‑Terminus) LS‑C353001
Species: Human, Bovine, Pig, Chicken, Zebrafish
Applications: Western blot
EZR / Ezrin Antibody - Western blot analysis of lysates from HeLa, HepG2, and K562 cells, using Ezrin Antibody. The lane on the right is blocked with the synthesized peptide.
EZR / Ezrin Antibody (aa111‑160) LS‑C198940
Species: Human, Mouse, Rat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
EZR / Ezrin ELISA Kit
Human EZR / Ezrin (Sandwich ELISA) ELISA Kit - LS-F9292
Reactivity: Human
Range: 0.313-20 ng/ml
See all EZR / Ezrin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
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