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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C352175-100 100 µl (1 mg/ml) $245 
ETV6 / TEL Antibody - Immunohistochemical analysis of TEL staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ETV6 / TEL Antibody - Immunofluorescent analysis of TEL staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ETV6 / TEL Antibody - Western blot analysis of TEL expression in CCRFCEM (A); Jurkat (B); K562 (C) whole cell lysates.
ETV6 / TEL Antibody - Immunohistochemical analysis of TEL staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ETV6 / TEL Antibody - Immunofluorescent analysis of TEL staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ETV6 / TEL Antibody - Western blot analysis of TEL expression in CCRFCEM (A); Jurkat (B); K562 (C) whole cell lysates.
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ETV6 / TEL Antibody (aa 396‑435) LS‑C352175

ETV6 / TEL Antibody (aa 396‑435) LS‑C352175

Antibody:
Rabbit Polyclonal to Human ETV6 / TEL
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine, Chicken, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$245 
LS-C352175-100
100 µl
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human ETV6 / TEL
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine, Chicken, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
TEL antibody LS-C352175 is an unconjugated rabbit polyclonal antibody to TEL (ETV6 ) from human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB.
Target
Human ETV6 / TEL
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human TEL.
Blocking Peptide
LS-E43727 - Lyophilized - 1 mg - $145.00
Immunizing peptide used to generate LS-C352175. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa 396-435
Specificity
Recognizes endogenous levels of TEL protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ETV6 / TEL
P41212 NM_001987 NP_001978.1

Publications (0)

Reviews (0)

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Requested From: United States
Date Requested: 10/17/2019