Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Locations
Orders Processing,
Shipping & Receiving,
Warehouse
2 Shaker Rd Suites
B001/B101
Shirley, MA 01464
Production Lab
Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036
Telephone Numbers
Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-B720-50 | 50 µg (1.2 mg/ml) | $515 |
![ESET / SETDB1 Antibody - Anti-ESET Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-ESET antibody to detect human ESET present in a 293 whole cell lysate. ~30 ug of lysate was loaded per lane for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kD is detected. Peptide competition (not shown) blocks staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/ihc-plus-eset-setdb1-antibody-ls-b720/62872_184052.jpg)
1 of 2
2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human ESET / SETDB1 Antibody (IHC, WB) LS‑B720
IHC‑plus™ Polyclonal Rabbit anti‑Human ESET / SETDB1 Antibody (IHC, WB) LS‑B720
Note: This antibody replaces LS-C18831
Antibody:
ESET / SETDB1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
ESET / SETDB1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
SETDB1 antibody LS-B720 is an unconjugated rabbit polyclonal antibody to human SETDB1 (ESET). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Target
Human ESET / SETDB1
Synonyms
SETDB1 | H3-K9-HMTase4 | ESET | KMT1E | H3-K9-HMTase 4 | KG1T | SET domain, bifurcated 1 | SET domain bifurcated 1 | KIAA0067 | Lysine N-methyltransferase 1E
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1050-1075 of human ESET.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:1000 - 1:2500)
- ELISA (1:10000 - 1:50000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B720 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B720 was determined to be 5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ESET / SETDB1
Validation
Anti-SETDB1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SETDB1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ ESET / SETDB1 Antibody for IHC, WB/Western, ELISA LS-B720 has an LSBio Rating of
Publications (4.3)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (4)
Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids. Harte PJ, Wu W, Carrasquillo MM, Matera AG. Cytogenetics and cell genetics. 1999 84:83-6.
SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins. Schultz DC, Ayyanathan K, Negorev D, Maul GG, Rauscher FJ. Genes & development. 2002 16:919-32.
mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression. Wang H, An W, Cao R, Xia L, Erdjument-Bromage H, Chatton B, Tempst P, Roeder RG, Zhang Y. Molecular cell. 2003 12:475-87.
Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly. Sarraf SA, Stancheva I. Molecular cell. 2004 15:595-605.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Non-Human Primate
Applications:
ICC, Immunofluorescence, Western blot
Source:
E. coli
Tag:
GST, N-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
Date Requested: 4/27/2024
