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Antibodies > ERGIC-53 / LMAN1 > LS-C756580
 
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Catalog Number Size Price
LS-C756580-10 10 µg $318 
LS-C756580-100 100 µg $470 
ERGIC-53 / LMAN1 Antibody - IHC analysis of LMAN1 using anti-LMAN1 antibody. LMAN1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-LMAN1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ERGIC-53 / LMAN1 Antibody - IHC analysis of LMAN1 using anti-LMAN1 antibody. LMAN1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-LMAN1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ERGIC-53 / LMAN1 Antibody - IHC analysis of LMAN1 using anti-LMAN1 antibody. LMAN1 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-LMAN1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ERGIC-53 / LMAN1 Antibody - IHC analysis of LMAN1 using anti-LMAN1 antibody. LMAN1 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-LMAN1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ERGIC-53 / LMAN1 Antibody - IHC analysis of LMAN1 using anti-LMAN1 antibody. LMAN1 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-LMAN1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ERGIC-53 / LMAN1 Antibody - Western blot analysis of LMAN1 using anti-LMAN1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysate,Lane 2: human A431 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMAN1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LMAN1 at approximately 58KD. The expected band size for LMAN1 is at 58KD.
ERGIC-53 / LMAN1 Antibody - IHC analysis of LMAN1 using anti-LMAN1 antibody. LMAN1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-LMAN1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ERGIC-53 / LMAN1 Antibody - IHC analysis of LMAN1 using anti-LMAN1 antibody. LMAN1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-LMAN1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ERGIC-53 / LMAN1 Antibody - IHC analysis of LMAN1 using anti-LMAN1 antibody. LMAN1 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-LMAN1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ERGIC-53 / LMAN1 Antibody - IHC analysis of LMAN1 using anti-LMAN1 antibody. LMAN1 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-LMAN1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ERGIC-53 / LMAN1 Antibody - IHC analysis of LMAN1 using anti-LMAN1 antibody. LMAN1 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-LMAN1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ERGIC-53 / LMAN1 Antibody - Western blot analysis of LMAN1 using anti-LMAN1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysate,Lane 2: human A431 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMAN1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LMAN1 at approximately 58KD. The expected band size for LMAN1 is at 58KD.
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Polyclonal Rabbit anti‑Human ERGIC‑53 / LMAN1 Antibody (IHC, WB) LS‑C756580

Polyclonal Rabbit anti‑Human ERGIC‑53 / LMAN1 Antibody (IHC, WB) LS‑C756580

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Antibody:
ERGIC-53 / LMAN1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756580-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ERGIC-53 / LMAN1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
LMAN1 antibody LS-C756580 is an unconjugated rabbit polyclonal antibody to LMAN1 (ERGIC-53) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Target
Human ERGIC-53 / LMAN1
Synonyms
LMAN1 | ERGIC53 | F5F8D | gp58 | ERGIC-53 | FMFD1 | Lectin mannose-binding 1 | MCFD1 | MR60 | Protein ERGIC-53 | Lectin, mannose-binding, 1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human LMAN1 recombinant protein (Position: K96-M458).
Specificity
No cross reactivity with other proteins.
Applications
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ERGIC-53 / LMAN1
P49257 NM_005570 NP_005561.1

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Featured Products

ERGIC-53 / LMAN1 Antibody - Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ERGIC‑53 / LMAN1 Antibody (aa36‑85) LS‑C80907
Species: Human
Applications: Western blot
ERGIC-53 / LMAN1 Antibody - IHC of paraffin-embedded Human pancreas tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
ERGIC‑53 / LMAN1 Antibody (clone OTI1A8) LS‑C337181
Species: Human, Monkey, Rat, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
ERGIC-53 / LMAN1 Antibody - IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
ERGIC‑53 / LMAN1 Antibody (clone OTI1C5) LS‑C337213
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
UTRN / Utrophin ELISA Kit
Mouse UTRN / Utrophin (Sandwich ELISA) ELISA Kit - LS-F8606
Reactivity: Mouse
Range: 0.78-50 ng/ml
TFPI2 ELISA Kit
Mouse TFPI2 (Sandwich ELISA) ELISA Kit - LS-F55163
Reactivity: Mouse
Range: 0.312-20 ng/ml
GLP1R / GLP-1 Receptor ELISA Kit
Mouse GLP1R / GLP-1 Receptor (Sandwich ELISA) ELISA Kit - LS-F6781
Reactivity: Mouse
Range: 0.156-10 ng/ml
See all ERGIC-53 / LMAN1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
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