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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ERGIC-53 / LMAN1 Antibody (clone 2B10) IHC-plus™ LS-B14773

Note: This antibody replaces LS-C172367
LMAN1 antibody LS-B14773 is an unconjugated mouse monoclonal antibody to LMAN1 (ERGIC-53) from human and monkey. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B14773-100
100 µl
$425
Description
LMAN1 antibody LS-B14773 is an unconjugated mouse monoclonal antibody to LMAN1 (ERGIC-53) from human and monkey. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ERGIC-53 / LMAN1
Host
Mouse
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
2B10
Conjugations
Unconjugated
Purification
affinity chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:200 - 1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length human recombinant protein of human LMAN1 (NP_005561) produced in HEK293T cell.
Specificity
Human ERGIC-53 / LMAN1
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About ERGIC-53 / LMAN1
P49257 NM_005570 NP_005561.1

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Applications: Western blot
IHC of paraffin-embedded Human pancreas tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
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IHC of paraffin-embedded Human Kidney tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
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Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:50.
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Applications: IHC, IHC - Paraffin, Western blot

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Images

Immunohistochemistry - Paraffin

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Anti-LMAN1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1.
Anti-LMAN1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.

Immunohistochemistry - Paraffin

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Anti-LMAN1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1.
Anti-LMAN1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.

Immunohistochemistry - Paraffin

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Anti-LMAN1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1.
Anti-LMAN1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.

Immunohistochemistry - Paraffin

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Anti-LMAN1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1.
Anti-LMAN1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.

Requested From: United States
Date Requested: 5/20/2019
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