Monoclonal Mouse anti‑Human EPCAM Antibody (clone SPM491, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C390603
Monoclonal Mouse anti‑Human EPCAM Antibody (clone SPM491, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C390603
Antibody:
EPCAM Mouse anti-Human Monoclonal (Azide-free, BSA-free) (SPM491) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Format:
Unconjugated, Azide-free, BSA-free
Overview
Antibody:
EPCAM Mouse anti-Human Monoclonal (Azide-free, BSA-free) (SPM491) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Format:
Unconjugated, Azide-free, BSA-free
Specifications
Description
EPCAM antibody LS-C390603 is an unconjugated mouse monoclonal antibody to human EPCAM. Validated for Flow, IF, IHC and WB.
Target
Human EPCAM
Synonyms
EPCAM | 323/A3 | ACSTD1 | 17-1A | CD326 | EGP | EGP34 | Epithelial glycoprotein | GA733-2 | HNPCC8 | Ep-CAM | ESA | HEGP314 | KS 1/4 antigen | KS1/4 | KSA | M1S2 | MIC18 | MK-1 | MH99 | TROP1 | TACST-1 | TACSTD1 | CD326 antigen | CO-17A | DIAR5 | EGP-2 | EGP314 | EGP40 | Epithelial glycoprotein 314 | HEA125 | Ly74 | M4S1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free, BSA-free.
Also available
Unmodified.
Immunogen
Recombinant Full-length Human Protein.
Specificity
Human TACSTD1 / EPCAM
Applications
- IHC
- IHC - Paraffin (0.25 - 0.5 µg/ml)
- Immunofluorescence (1 - 2 µg/ml)
- Western blot (0.5 - 1 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. Optimal dilution of the EpCAM antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Mouse
Applications:
IHC, IHC - Frozen, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human
Applications:
IHC - Frozen, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Species:
Pig
Applications:
Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/28/2024