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Catalog Number	Size	Price
LS-C331216-20	20 µl	$287
LS-C331216-100	100 µl	$431

ENO1 / Alpha Enolase Antibody - Immunofluorescence analysis of HeLa cells using ENO1 antibody. Blue: DAPI for nuclear staining.

ENO1 / Alpha Enolase Antibody - Western blot analysis of extracts from normal (control) and ENO1 knockout (KO) HeLa cells, using ENO1 antibodyat 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.

ENO1 / Alpha Enolase Antibody - Western blot analysis of extracts of various cell lines, using ENO1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

ENO1 / Alpha Enolase Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ENO1 antibody. Western blot was performed from the immunoprecipitate using ENO1 antibodyat a dilition of 1:1000.

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Polyclonal Rabbit anti‑Human ENO1 / Alpha Enolase Antibody (IHC, IF, WB) LS‑C331216

Name: Anti-ENO1 / Alpha Enolase Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C331216-20
Price: 287.0000 USD
Availability: InStock

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Antibody:

ENO1 / Alpha Enolase Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB, IP

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$287

LS-C331216-20

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ENO1 / Alpha Enolase Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB, IP

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

Alpha Enolase antibody LS-C331216 is an unconjugated rabbit polyclonal antibody to Alpha Enolase (ENO1) from human. It is reactive with human and mouse. Validated for IF, IHC, IP and WB.

Target

Human ENO1 / Alpha Enolase

Synonyms

See All ENO1 / Alpha Enolase Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity purified

Modifications

Unmodified

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-434 of human ENO1 (NP_001419.1). MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVG

Specificity

Human ENO1 / Alpha Enolase

Applications

IHC (1:50 - 1:200)
Immunofluorescence (1:50 - 1:100)
Western blot (1:500 - 1:2000)
Immunoprecipitation (1:50 - 1:200)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

The predicted MW is 36kDa/47kDa, while the observed MW by Western blot was 47kDa.

Presentation

PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About ENO1 / Alpha Enolase