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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF2S1 Antibody (clone 1H1) LS-C115391

EIF2S1 antibody LS-C115391 is an unconjugated mouse monoclonal antibody to EIF2S1. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C115391-100
100 µl (1 mg/ml)
$345
Description
EIF2S1 antibody LS-C115391 is an unconjugated mouse monoclonal antibody to EIF2S1. Validated for Flow, IHC and WB.
Target
Human EIF2S1
Host
Mouse
Clonality
IgG1 Monoclonal
Clone
1H1
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
EIF2S1 antibody was raised against full length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell
Specificity
Human EIF2S1
Usage
Concentration: 0.51 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About EIF2S1
P05198 NM_004094 NP_004085.1

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Images

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-EIF2S1 mouse monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-EIF2S1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.

Flow Cytometry

Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-EIF2S1 mouse monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-EIF2S1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.

Flow Cytometry

Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-EIF2S1 mouse monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-EIF2S1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.

Flow Cytometry

Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-EIF2S1 mouse monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-EIF2S1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.

Flow Cytometry

Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Requested From: United States
Date Requested: 5/24/2019
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