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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-C482262-50 50 µl (1 mg/ml) $255 
LS-C482262-100 100 µl (1 mg/ml) $295 
LS-C482262-200 200 µl (1 mg/ml) $375 
EGLN1 / PHD2 Antibody - Immunohistochemical analysis of EGLN1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
EGLN1 / PHD2 Antibody - Immunofluorescent analysis of EGLN1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
EGLN1 / PHD2 Antibody - Western blot analysis of EGLN1 expression in SHSY5Y (A); HeLa (B); COS7 (C) whole cell lysates.
EGLN1 / PHD2 Antibody - Immunohistochemical analysis of EGLN1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
EGLN1 / PHD2 Antibody - Immunofluorescent analysis of EGLN1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
EGLN1 / PHD2 Antibody - Western blot analysis of EGLN1 expression in SHSY5Y (A); HeLa (B); COS7 (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human EGLN1 / PHD2 Antibody (IHC, IF, WB) LS‑C482262

Polyclonal Rabbit anti‑Human EGLN1 / PHD2 Antibody (IHC, IF, WB) LS‑C482262

Antibody:
Rabbit Polyclonal to Human EGLN1 / PHD2
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255 
LS-C482262-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human EGLN1 / PHD2
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PHD2 antibody LS-C482262 is an unconjugated rabbit polyclonal antibody to PHD2 (EGLN1) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human EGLN1 / PHD2
Synonyms
EGLN1 | C1orf12 | EGL9 | ECYT3 | Egl nine-like protein 1 | HIFPH2 | HIF prolyl hydroxylase 2 | HIF-PH2 | HIF-prolyl hydroxylase 2 | ZMYND6 | PHD2 | SM20 | EGL nine (C.elegans) homolog 1 | Egl nine homolog 1 | HPH-2 | HPH2 | SM-20
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:200)
  • ICC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant full length human EGLN1.
Specificity
Recognizes endogenous levels of EGLN1 protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EGLN1 / PHD2
Q9GZT9 NM_022051 NP_071334.1

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Requested From: United States
Date Requested: 8/5/2020