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Catalog Number Size Price
LS-C810332-100 100 µl (1 mg/ml) $379 
LS-C810332-200 200 µl (1 mg/ml) $421 
EFNB1 / Ephrin B1 Antibody - 1:100 staining rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
EFNB1 / Ephrin B1 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
EFNB1 / Ephrin B1 Antibody - Western blot analysis of EFNB1/2 expression in EGF treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
EFNB1 / Ephrin B1 Antibody - 1:100 staining rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
EFNB1 / Ephrin B1 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
EFNB1 / Ephrin B1 Antibody - Western blot analysis of EFNB1/2 expression in EGF treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human EFNB1 / Ephrin B1 Antibody (IHC, IF, WB) LS‑C810332

Polyclonal Rabbit anti‑Human EFNB1 / Ephrin B1 Antibody (IHC, IF, WB) LS‑C810332

Antibody:
EFNB1 / Ephrin B1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C810332-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
EFNB1 / Ephrin B1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Ephrin B1 antibody LS-C810332 is an unconjugated rabbit polyclonal antibody to Ephrin B1 (EFNB1) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human EFNB1 / Ephrin B1
Synonyms
EFNB1 | CFNS | EFL3 | ELK ligand | Ephrin B1 | Ephrin-B1 | Elk-L | EFL-3 | CFND | EPLG2 | LERK-2 | LERK2 | Ligand of eph-related kinase 2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human EFNB1/2.
Specificity
EFNB1/2 Antibody detects endogenous levels of total EFNB1/2.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EFNB1 / Ephrin B1
P98172 NM_004429 NP_004420.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024