Pref-1 antibody LS-C9685 is an unconjugated rabbit polyclonal antibody to mouse Pref-1 (DLK1). Validated for ELISA and WB.
Mouse DLK1 / Pref-1
(tested or 100% immunogen sequence identity)
- Western blot (1 - 5 µg/ml)
- ELISA (1:10000 - 1:50000)
DLK1 / Pref-1 antibody was raised against synthetic peptide consisting of 16aa sequence near the cytoplasmic, C-terminus of mouse Pref-1 (KLH coupled).
Recognizes mouse Preadipocyte Factor 1. Because the epitope is located near the cytoplasmic C-terminus, will not detect the ectodomain/soluble forms of Pref-1. Species sequence Homology: Human, rat: 93%; bovine: 87%.
Suitable for use in ELISA and Western Blot. Western Blot: 1-5 ug/ml using ECL. ELISA: 1:10000-1:50000. Coat ELISA plates with 10-100 ng of control peptide/well.
PBS, 0.2% BSA, 40% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.