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Catalog Number Size Price
LS-C820993-100 100 µg (1 mg/ml) $533 
Dibromo-tyrosine Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 9F12. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Dibromo-tyrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Dibromo-tyrosine Antibody - Western Blot analysis of 3, 5-Dibromotyrosine-BSA Conjugate showing detection of 67 kDa Dibromo-tyrosine protein using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 9F12. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: Nitrosylated-BSA. Lane 4: Dibromotyrosine-BSA. Load: 1 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Dibromo-tyrosine Antibody - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Dibromo-tyrosine protein using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 9F12. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
Dibromo-tyrosine Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 9F12. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Dibromo-tyrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Dibromo-tyrosine Antibody - Western Blot analysis of 3, 5-Dibromotyrosine-BSA Conjugate showing detection of 67 kDa Dibromo-tyrosine protein using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 9F12. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: Nitrosylated-BSA. Lane 4: Dibromotyrosine-BSA. Load: 1 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Dibromo-tyrosine Antibody - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Dibromo-tyrosine protein using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 9F12. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
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Monoclonal Mouse anti‑All species Dibromo‑tyrosine Antibody (clone 9F12, PerCP, IF, WB) LS‑C820993

Monoclonal Mouse anti‑All species Dibromo‑tyrosine Antibody (clone 9F12, PerCP, IF, WB) LS‑C820993

Antibody:
Dibromo-tyrosine Mouse Monoclonal (PerCP) (9F12) Antibody
Application:
IF, WB, ELISA
Reactivity:
All species
Format:
Peridinin-chlorophyll-protein, Unmodified
Other formats:
Price
Catalog Number
$533
LS-C820993-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Dibromo-tyrosine Mouse Monoclonal (PerCP) (9F12) Antibody
Application:
IF, WB, ELISA
Reactivity:
All species
Format:
Peridinin-chlorophyll-protein, Unmodified
Other formats:

Specifications

Description
Dibromo-tyrosine antibody LS-C820993 is a PerCP-conjugated mouse monoclonal antibody to all species Dibromo-tyrosine. Validated for ELISA, IF and WB.
Target
All species Dibromo-tyrosine
Host
Mouse
Reactivity
All species
Clonality
IgG1 Monoclonal
Clone
9F12
Conjugations
Peridinin-chlorophyll-protein. Also available conjugated with Atto 390, Atto 488, Atto 594, APC, Biotin, FITC, RPE.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic 3,5-Dibromotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity
Specific for 3,5-Dibromo-tyrosine. Does not cross-react with Nitrotyrosine.
Applications
  • Immunofluorescence (1:50)
  • Western blot (1:1000)
  • ELISA (1:1000)
  • Applications tested for the base form of this product only
Usage
A 1:1000 dilution of the antibody was sufficient for detection of 3,5-Dibromotyrosine in 1 µg of 3, 5-Dibromotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody. Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 5/18/2024