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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DIAPH2 Antibody (aa1085-1101) LS-C19007

Best Seller
Note: This antibody replaces LS-C64718
DIAPH2 antibody LS-C19007 is an unconjugated rabbit polyclonal antibody to human DIAPH2. Validated for ELISA and WB. Cited in 1 publication.
Catalog
Size
Price
LS-C19007-100
100 µg (1.4 mg/ml)
$425
Description
DIAPH2 antibody LS-C19007 is an unconjugated rabbit polyclonal antibody to human DIAPH2. Validated for ELISA and WB. Cited in 1 publication.
Target
Human DIAPH2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:6000)
  • ELISA (1:12000 - 1:60000)
Failed Applications
  • IHC - Paraffin
Immunogen
DIAPH2 antibody was raised against synthetic peptide from human DIAPH2.
Epitope
aa1085-1101
Specificity
aa 1085-1101 of Human DIA-2.
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 125 kD in size corresponding to DIA-2 by western blotting in the appropriate cell lysate or extract. Alternate splice variants have been described for this protein. This antibody will only react with DIA-156 (isoform 1 of DIA-2).
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
About DIAPH2
O60879 NM_006729 NP_006720.1

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Publications (1)

1
Diaphanous formin mDia2 regulates CENP-A levels at centromeres. Liu C, Mao Y. The Journal of cell biology. 2016 213:415-24. (WB; Human) [Full Text Article] [PubMed:27185834] [PMC:PMC4878093]

Customer Reviews (0)


Images

Western blot

Anti-DIA-2 Antibody - Western Blot. Western blot of Affinity Purified anti-DIA-2 antibody shows detection of a 132-kD band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
Anti-DIA-2 Antibody - Western Blot. Western blot of Affinity Purified anti-DIA-2 antibody shows detection of a 132-kD band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.

Western blot

Anti-DIA-2 Antibody - Western Blot. Western blot of Affinity Purified anti-DIA-2 antibody shows detection of a 132-kD band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
Anti-DIA-2 Antibody - Western Blot. Western blot of Affinity Purified anti-DIA-2 antibody shows detection of a 132-kD band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.

Western blot

Anti-DIA-2 Antibody - Western Blot. Western blot of Affinity Purified anti-DIA-2 antibody shows detection of a 132-kD band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
Anti-DIA-2 Antibody - Western Blot. Western blot of Affinity Purified anti-DIA-2 antibody shows detection of a 132-kD band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.

Western blot

Anti-DIA-2 Antibody - Western Blot. Western blot of Affinity Purified anti-DIA-2 antibody shows detection of a 132-kD band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
Anti-DIA-2 Antibody - Western Blot. Western blot of Affinity Purified anti-DIA-2 antibody shows detection of a 132-kD band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.

Requested From: United States
Date Requested: 4/23/2019
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