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Catalog Number Size Price
LS-C814794-100 100 µl $412 
DEK Antibody - Immunofluorescence staining of DEK in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human DEK polyclonal antibody (dilution ratio 1:5000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
DEK Antibody - Anti-DEK rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 50 kDa.
DEK Antibody - DEK was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. 1 uL anti-DEK rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-DEK rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa.
DEK Antibody - Immunofluorescence staining of DEK in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human DEK polyclonal antibody (dilution ratio 1:5000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
DEK Antibody - Anti-DEK rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 50 kDa.
DEK Antibody - DEK was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. 1 uL anti-DEK rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-DEK rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa.
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Polyclonal Rabbit anti‑Human DEK Antibody (Preservative Free, IF, WB) LS‑C814794

Polyclonal Rabbit anti‑Human DEK Antibody (Preservative Free, IF, WB) LS‑C814794

Antibody:
DEK Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$412
LS-C814794-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DEK Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Preservative Free

Specifications

Description
DEK antibody LS-C814794 is an unconjugated rabbit polyclonal antibody to human DEK. Validated for ICC, IF, IP and WB.
Target
Human DEK
Synonyms
DEK | D6S231E | DEK oncogene (DNA binding) | Protein DEK | DEK oncogene
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Preservative Free
Immunogen
A synthetic peptide corresponding to the center region of the Human DEK
Specificity
Human DEK
Applications
  • ICC (1:1500 - 1:50000)
  • Immunofluorescence (1:1500 - 1:50000)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DEK
P35659 NM_003472 NP_003463.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/30/2024