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Antibodies > DDX39B / UAP56 > LS-C761213
 
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Catalog Number Size Price
LS-C761213-50 50 µl (1 mg/ml) $296 
LS-C761213-100 100 µl (1 mg/ml) $341 
LS-C761213-200 200 µl (1 mg/ml) $421 
DDX39B / UAP56 Antibody - Immunohistochemical analysis of DDX39B staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
DDX39B / UAP56 Antibody - Immunofluorescent analysis of DDX39B staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
DDX39B / UAP56 Antibody - Western blot analysis of DDX39B expression in HepG2 (A), K562 (B), Jurkat (C), NIH3T3 (D), MCF7 (E) whole cell lysates.
DDX39B / UAP56 Antibody - Immunohistochemical analysis of DDX39B staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
DDX39B / UAP56 Antibody - Immunofluorescent analysis of DDX39B staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
DDX39B / UAP56 Antibody - Western blot analysis of DDX39B expression in HepG2 (A), K562 (B), Jurkat (C), NIH3T3 (D), MCF7 (E) whole cell lysates.
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Monoclonal Mouse anti‑Human DDX39B / UAP56 Antibody (IHC, IF, WB) LS‑C761213

Monoclonal Mouse anti‑Human DDX39B / UAP56 Antibody (IHC, IF, WB) LS‑C761213

Submit Review LSBio Guarantee
Antibody:
DDX39B / UAP56 Mouse anti-Human Monoclonal Antibody
Application:
IHC, ICC, IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$296
LS-C761213-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DDX39B / UAP56 Mouse anti-Human Monoclonal Antibody
Application:
IHC, ICC, IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
UAP56 antibody LS-C761213 is an unconjugated mouse monoclonal antibody to UAP56 (DDX39B) from human. It is reactive with human and mouse. Validated for Flow, ICC, IF, IHC and WB.
Target
Human DDX39B / UAP56
Synonyms
DDX39B | BAT1 | DEAD box protein UAP56 | HLA-B associated transcript 1 | UAP56 | ATP-dependent RNA helicase p47 | D6S81E | Spliceosome RNA helicase BAT1
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant fragment of human DDX39B expressed in E. Coli
Specificity
Recognizes endogenous levels of DDX39B protein.
Applications
  • IHC (1:100 - 1:500)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Flow Cytometry (1:100 - 1:200)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.42% Potassium Phosphate, pH 7.3, 0.87% NaCl, 0.01% Sodium Azide, 30% Glycerol
Storage
Upon delivery, aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DDX39B / UAP56
Q13838 NM_004640 NP_004631.1

Publications (0)

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IHC‑plus™ DDX39B / UAP56 Antibody (clone 2E4) LS‑B12960
Species: Human, Rat, Dog
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DDX39B / UAP56 Antibody - IHC of paraffin-embedded Human prostate tissue using anti-BAT1 mouse monoclonal antibody. (Dilution 1:50).
DDX39B / UAP56 Antibody (clone OTI2C5) LS‑C115216
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
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Reactivity: Rat
Range: 20-320 ng/ml
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See all DDX39B / UAP56 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/30/2024
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