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Antibodies > DDR2 > LS-C313017
 
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Catalog Number Size Price
LS-C313017-10 10 µg $318 
LS-C313017-100 100 µg (0.5 mg/ml) $470 
DDR2 Antibody - Western blot analysis of DDR2 using anti-DDR2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HT1080 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human U2OS whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. Specific bands were detected for DDR2 at approximately 97,120-140KD. The expected band size for DDR2 is at 97KD.
DDR2 Antibody - WB of DDR2 antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant human DDR2, 41.2KD. (162aa tag+Y656-E855). Lane 1: Recombinant Human DDR2 Protein 10ng. Lane 2: Recombinant Human DDR2 Protein 5ng. Lane 3: Recombinant Human DDR2 Protein 2.5ng.
DDR2 Antibody - Western blot analysis of DDR2 using anti-DDR2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HT1080 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human U2OS whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. Specific bands were detected for DDR2 at approximately 97,120-140KD. The expected band size for DDR2 is at 97KD.
DDR2 Antibody - WB of DDR2 antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant human DDR2, 41.2KD. (162aa tag+Y656-E855). Lane 1: Recombinant Human DDR2 Protein 10ng. Lane 2: Recombinant Human DDR2 Protein 5ng. Lane 3: Recombinant Human DDR2 Protein 2.5ng.
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Polyclonal Rabbit anti‑Human DDR2 Antibody (aa834‑855, WB) LS‑C313017

Polyclonal Rabbit anti‑Human DDR2 Antibody (aa834‑855, WB) LS‑C313017

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Note: This antibody replaces LS-C388343
Antibody:
DDR2 Rabbit anti-Human Polyclonal (aa834-855) Antibody
Application:
WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313017-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DDR2 Rabbit anti-Human Polyclonal (aa834-855) Antibody
Application:
WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
DDR2 antibody LS-C313017 is an unconjugated rabbit polyclonal antibody to DDR2 (aa834-855) from human. It is reactive with human and monkey. Validated for WB.
Target
Human DDR2
Synonyms
DDR2 | CD167b antigen | Discoidin domain receptor 2 | Hydroxyaryl-protein kinase | MIG20a | Tyrosine-protein kinase TYRO10 | NTRKR3 | TYRO10 | Tyrosylprotein kinase
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human DDR2(834-855 aa RDTKNRPSFQEIHLLLLQQGDE), different from the related mouse sequence by three amino acids.
Epitope
aa834-855
Specificity
Detected in osteocytes, osteoblastic cells in subchondral bone, bone lining cells, tibia and cartilage (at protein level). Detected at high levels in heart and lung, and at low levels in brain, placenta, liver, skeletal muscle, pancreas, and kidney. .
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DDR2
Q16832 NM_006182 NP_006173.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/4/2024
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