Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
206-374-1102
Fax:
206-577-4565
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C18970-100 100 µl (85 mg/ml) $498 
DDB2 Antibody - Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.

Polyclonal Rabbit anti‑Human DDB2 Antibody (aa419‑427, IHC, WB) LS‑C18970

Polyclonal Rabbit anti‑Human DDB2 Antibody (aa419‑427, IHC, WB) LS‑C18970

Antibody:
DDB2 Rabbit anti-Human Polyclonal (aa419-427) Antibody
Application:
IHC, WB, IP, ELISA, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C18970-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DDB2 Rabbit anti-Human Polyclonal (aa419-427) Antibody
Application:
IHC, WB, IP, ELISA, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DDB2 antibody LS-C18970 is an unconjugated rabbit polyclonal antibody to human DDB2 (aa419-427). Validated for DB, ELISA, IHC, IP and WB. Cited in 2 publications.
Target
Human DDB2
Synonyms
DDB2 | DNA damage-binding protein 2 | DDB p48 subunit | UV-DDB 2 | DDBB | UV-DDB2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated followed by sterile filtration
Modifications
Unmodified
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 419-427 of Human DDB2 (C-terminal) coupled to KLH.
Epitope
aa419-427
Specificity
Reacts with human DDB2. Cross reactivity with DDB2 from other sources is not known.
Applications
  • IHC
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA (1:2000 - 1:10000)
  • Dot Blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This antibody reacts with human DDB2 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using 293T, and others). Coimmunoprecipitation of related proteins has not been tested. A 47.8 kD band corresponding to human DDB2 is detected. Most cell lines expressing DDB2 can be used as a positive control. Researchers should determine optimal titers for other applications.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DDB2
Q92466 NM_000107 NP_000098.1

LSBio Ratings

DDB2 Antibody (aa419-427) for IHC, WB/Western, IP, ELISA LS-C18970 has an LSBio Rating of
Publications (4.1)

Publications (2)

The naturally occurring mutants of DDB are impaired in stimulating nuclear import of the p125 subunit and E2F1-activated transcription. Shiyanov P, Hayes SA, Donepudi M, Nichols AF, Linn S, Slagle BL, Raychaudhuri P. Molecular and cellular biology. 1999 19:4935-43.

Cullin 4A associates with the UV-damaged DNA-binding protein DDB. Shiyanov P, Nag A, Raychaudhuri P. The Journal of biological chemistry. 1999 274:35309-12.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022