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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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DBT / E2 Antibody (aa83‑132, FITC) LS‑C455387
E2 antibody LS-C455387 is an FITC-conjugated rabbit polyclonal antibody to E2 (DBT) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C455387-100
100 µl (1 mg/ml)
$385

Popular DBT / E2 Products

Anti-DBT / E2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBT.
Species: Human
Applications: Western blot, Flow Cytometry
Anti-DBT / E2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Anti-DBT / E2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Anti-DBT / E2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot

Product Description

E2 antibody LS-C455387 is an FITC-conjugated rabbit polyclonal antibody to E2 (DBT) from human, mouse, rat and other species. Validated for WB.
About DBT / E2
The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. P11182 NM_001918 NP_001909.3

DBT Antibody, BCKAD E2 subunit Antibody, BCKAD-E2 Antibody, BCKADE2 Antibody, Dihydrolipoyl transacylase Antibody, E2 Antibody, E2B Antibody, BCATE2 Antibody


Specifications

Target
Human DBT / E2
Host
Rabbit
Reactivity
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Bat, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Fluorescein. Also available Unconjugated or conjugated with Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
DBT / E2 antibody was raised against synthetic peptide located between aa83-132 of human DBT (Q5VVL7). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Lizard (100%); Galago, Bat, Xenopus, Trout, Stickleback, Medaka, Pufferfish, Zebrafish (92%); Nematode (91%); Turkey, Chicken (85%); Drosophila (83%).
Epitope
aa83-132
Specificity
Human DBT / E2
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS.
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Western blot

Western blot of Human Fetal Kidney. DBT antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human Fetal Kidney. DBT antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human Fetal Kidney. DBT antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human Fetal Kidney. DBT antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human Fetal Kidney. DBT antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human Fetal Kidney. DBT antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human Fetal Kidney. DBT antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human Fetal Kidney. DBT antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 7/21/2018

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