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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-C392273-100 100 µg (1 mg/ml) $465 
Cytokeratin 1+8 Antibody - Formalin-fixed, paraffin-embedded human breast carcinoma stained with anti-Cytokeratin 18 antibody (SPM510).  This image was taken for the unmodified form of this product. Other forms have not been tested.

Cytokeratin 1+8 Antibody (clone SPM510, Azide‑free) LS‑C392273

Cytokeratin 1+8 Antibody (clone SPM510, Azide‑free) LS‑C392273

Antibody:
Mouse Monoclonal to Human Cytokeratin 1+8
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free
Other formats:
Price
Catalog Number
$465 
LS-C392273-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human Cytokeratin 1+8
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free
Other formats:

Specifications

Description
Cytokeratin 1+8 antibody LS-C392273 is an unconjugated mouse monoclonal antibody to human Cytokeratin 1+8. Validated for Flow, IF, IHC and WB.
Target
Human Cytokeratin 1+8
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat, Bovine, Dog, Hamster, Pig, Sheep
Clonality
IgG1,k Monoclonal
Clone
SPM510
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free. Also available Unmodified.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human breast cancer PMC 42 cells were used as the immunogen for this anti-Cytokeratin 18 antibody.
Specificity
Human Cytokeratin 1+8
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. The optimal dilution of the anti-Cytokeratin 18 antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Presentation
Sterile Solution PBS
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

Reviews (0)

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RIPK3 / RIP3 ELISA Kit
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Range: 0.156-10 ng/ml

Requested From: United States
Date Requested: 10/15/2019