Target
Mouse CYTIP
Synonyms
CYTIP | B3-1 | CASP | Cbp HE | CYTHIP | Cytohesin binder and regulator | Cytohesin binding protein HE | Cytohesin-binding protein HE | CYBR | Cytohesin-interacting protein | HE | PSCDBP
Reactivity
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
PSCDBP antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region in the C-terminal portion of mouse Cybr.
Specificity
A BLAST analysis was used to suggest cross-reactivity with Cybr from mouse and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
- Western blot (1:1000 - 1:10000)
- Immunoprecipitation (1:100)
- ELISA (1:5000 - 1:20000)
Usage
This antibody has been tested for use in ELISA and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kD in size corresponding to Cybr by western blotting after IP in the appropriate cell lysate or extract. To date, this antibody has not shown direct detection of endogenous or over-expressed Cybr protein, but is capable of immunoprecipitating the antigen from lysates. It is possible, but has not yet been confirmed, that this antibody recognizes a conformational epitope.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee