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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYP2J2 Antibody LS‑C416283
CYP2J2 antibody LS-C416283 is an unconjugated rabbit polyclonal antibody to human CYP2J2. Validated for ICC, IF, IHC and WB.
Catalog
Size
Price
LS-C416283-100
100 µg (1 mg/ml)
$305

Popular CYP2J2 Products

Immunohistochemical analysis of Cytochrome P450 2J2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemistry analysis of paraffin-embedded human heart tissue, using Cytochrome P450 2J2 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of CYP2J2 antibody
Species: Human, Monkey
Applications: IHC, Immunofluorescence, Western blot, ELISA
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP2J2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry

Product Description

CYP2J2 Antibody for IHC, ICC, IF/Immunofluorescence, WB/Western LS-C416283

Specifications

Target
Human CYP2J2
Synonyms
CYP2J2, Arachidonic acid epoxygenase, CYPIIJ2, Cytochrome p450 IIj2, CPJ2, Cytochrome P450 2J2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CYP2J2 antibody was raised against synthetic peptide from human Cytochrome P450 2J2.
Specificity
Cytochrome P450 2J2 Antibody detects endogenous levels of Cytochrome P450 2J2.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
About CYP2J2
This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools. P51589 NM_000775 NP_000766.2


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Images

Western blot

Western blot of HT29 cell lysate using Cytochrome P450 2J2 Antibody
Western blot of HT29 cell lysate using Cytochrome P450 2J2 Antibody

Western blot

Western blot of HT29 cell lysate using Cytochrome P450 2J2 Antibody
Western blot of HT29 cell lysate using Cytochrome P450 2J2 Antibody

Western blot

Western blot of HT29 cell lysate using Cytochrome P450 2J2 Antibody
Western blot of HT29 cell lysate using Cytochrome P450 2J2 Antibody

Western blot

Western blot of HT29 cell lysate using Cytochrome P450 2J2 Antibody
Western blot of HT29 cell lysate using Cytochrome P450 2J2 Antibody

Requested From: United States
Date Requested: 10/21/2018

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