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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYP2F / CYP2F1 Antibody IHC‑plus™ LS‑B10872
Note: This antibody replaces LS-C155481
CYP2F1 antibody LS-B10872 is an unconjugated rabbit polyclonal antibody to human CYP2F1 (CYP2F). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B10872-100
100 µl (0.69 mg/ml)
$425

Popular CYP2F / CYP2F1 Products

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CYP2F1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry of paraffin-embedded human lung using antibody at 1:100 dilution.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemistry of paraffin-embedded mouse lung tissue.
Species: Human, Mouse, Rat
Applications: IHC, Western blot
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

CYP2F1 antibody LS-B10872 is an unconjugated rabbit polyclonal antibody to human CYP2F1 (CYP2F). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CYP2F / CYP2F1
CYP2F / CYP2F1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to dehydrogenate 3-methylindole, an endogenous toxin derived from the fermentation of tryptophan, as well as xenobiotic substrates such as naphthalene and ethoxycoumarin. P24903 NM_000774 NP_000765.2

CYP2F1 Antibody, C2F1 Antibody, CYP2F Antibody, CYPIIF1 Antibody


Specifications

Target
Human CYP2F / CYP2F1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (7.5 µg/ml)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CYP2F / CYP2F1 antibody was raised against recombinant fragment corresponding to a region within amino acids 55 and 326 of CYP2F1 (SwissProt P24903).
Specificity
Human CYP2F1
Usage
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 7.5 µg/ml. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Presentation
0.1 M Tris-glycine, pH 7, 20% Glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-CYP2F / CYP2F1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10872 concentration 7.5 ug/ml.
Anti-CYP2F / CYP2F1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10872 concentration 7.5 ug/ml.

Western blot

Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE CYP2F1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE CYP2F1 antibody diluted at 1:1000

Immunohistochemistry

Anti-CYP2F / CYP2F1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10872 concentration 7.5 ug/ml.
Anti-CYP2F / CYP2F1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10872 concentration 7.5 ug/ml.

Western blot

Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE CYP2F1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE CYP2F1 antibody diluted at 1:1000

Immunohistochemistry

Anti-CYP2F / CYP2F1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10872 concentration 7.5 ug/ml.
Anti-CYP2F / CYP2F1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10872 concentration 7.5 ug/ml.

Western blot

Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE CYP2F1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE CYP2F1 antibody diluted at 1:1000

Immunohistochemistry

Anti-CYP2F / CYP2F1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10872 concentration 7.5 ug/ml.
Anti-CYP2F / CYP2F1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10872 concentration 7.5 ug/ml.

Western blot

Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE CYP2F1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE CYP2F1 antibody diluted at 1:1000

Requested From: United States
Date Requested: 9/25/2018

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