Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > CYP17 / CYP17A1 > LS-C756411
 
Print Page  
Catalog Number Size Price
LS-C756411-10 10 µg $318 
LS-C756411-100 100 µg $470 
CYP17 / CYP17A1 Antibody - IHC analysis of Cyp17a1 using anti-Cyp17a1 antibody. Cyp17a1 was detected in paraffin-embedded section of rat testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-Cyp17a1 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CYP17 / CYP17A1 Antibody - IHC analysis of Cyp17a1 using anti-Cyp17a1 antibody. Cyp17a1 was detected in paraffin-embedded section of mouse testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-Cyp17a1 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CYP17 / CYP17A1 Antibody - Western blot analysis of Cyp17a1 using anti-Cyp17a1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyp17a1 antigen affinity purified polyclonal antibody at 0.5 ?g/mL overnight at 4?C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyp17a1 at approximately 57KD. The expected band size for Cyp17a1 is at 57KD.
CYP17 / CYP17A1 Antibody - IHC analysis of Cyp17a1 using anti-Cyp17a1 antibody. Cyp17a1 was detected in paraffin-embedded section of rat testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-Cyp17a1 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CYP17 / CYP17A1 Antibody - IHC analysis of Cyp17a1 using anti-Cyp17a1 antibody. Cyp17a1 was detected in paraffin-embedded section of mouse testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-Cyp17a1 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CYP17 / CYP17A1 Antibody - Western blot analysis of Cyp17a1 using anti-Cyp17a1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyp17a1 antigen affinity purified polyclonal antibody at 0.5 ?g/mL overnight at 4?C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyp17a1 at approximately 57KD. The expected band size for Cyp17a1 is at 57KD.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Mouse CYP17 / CYP17A1 Antibody (IHC, WB) LS‑C756411

Polyclonal Rabbit anti‑Mouse CYP17 / CYP17A1 Antibody (IHC, WB) LS‑C756411

Submit Review LSBio Guarantee
Antibody:
CYP17 / CYP17A1 Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756411-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CYP17 / CYP17A1 Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CYP17A1 antibody LS-C756411 is an unconjugated rabbit polyclonal antibody to CYP17A1 (CYP17) from mouse. It is reactive with mouse and rat. Validated for ELISA, IHC and WB.
Target
Mouse CYP17 / CYP17A1
Synonyms
CYP17A1 | CYP17 | CYPXVII | CPT7 | Cytochrome P450-C17 | Cytochrome P450c17 | p450C17 | Steroid 17-alpha-monooxygenase | Cytochrome P450 17A1 | Cytochrome p450 XVIIA1 | S17AH
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived mouse Cyp17a1 recombinant protein (Position: Q80-R363).
Specificity
No cross reactivity with other proteins.
Applications
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYP17 / CYP17A1
P05093 NM_000102 NP_000093.1

Publications (0)

Submit a Publication for LS-C756411

Customer Reviews (0)

Submit a review for LS-C756411

Featured Products

CYP17 / CYP17A1 Antibody - Anti-CYP17 / CYP17A1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
IHC‑plus™ CYP17 / CYP17A1 Antibody (aa134‑144) LS‑B8295
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
CYP17 / CYP17A1 Antibody - Immunohistochemical analysis of Cytochrome P450 17A1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CYP17 / CYP17A1 Antibody (Internal) LS‑C352100
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
CYP17 / CYP17A1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ CYP17 / CYP17A1 Antibody (aa358‑388) LS‑B14227
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
CYP17 / CYP17A1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ CYP17 / CYP17A1 Antibody (aa1‑508) LS‑B13802
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
CYP17 / CYP17A1 Antibody - 1:50 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CYP17 / CYP17A1 Antibody LS‑C801327
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Product
CYP17 / CYP17A1 Antibody LS‑C393190
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
See all CYP17 / CYP17A1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com