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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CUX1 / CASP Antibody (clone 2A10) IHC‑plus™ LS‑B4309 Best Seller
Note: This antibody replaces LS-C104930, LS-C327143
CASP antibody LS-B4309 is an unconjugated mouse monoclonal antibody to human CASP (CUX1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4309-50
50 µg (0.5 mg/ml)
$485

Popular CUX1 / CASP Products

Immunohistochemical analysis of CUX1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
CUX1 / CUTL1 antibody LS-C107935 Western blot of Fetal Spleen lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Pig, Rabbit, Chicken, Xenopus
Applications: Western blot
CUX1 / CUTL1 antibody LS-C107935 Western blot of Fetal Spleen lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Pig, Rabbit, Chicken, Xenopus
Applications: Western blot
CUX1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

CASP antibody LS-B4309 is an unconjugated mouse monoclonal antibody to human CASP (CUX1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human CUX1 / CASP
Synonyms
CUX1, CASP, CDP1, Clox, COY1, CUTL1, CCAAT displacement protein, Cut homolog, CUX, CUT, GOLIM6, Homeobox protein cux-1, p100, p200, p75, Protein CASP, CDP, Cut-like homeobox 1, Nbla10317, p110
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [2A10]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CUX1 / CASP antibody was raised against cUTL1 (AAH25422, 521 a.a. ~ 621 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Usage
Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
Presentation
PBS, pH 7.4
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
About CUX1 / CASP
Probably has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Q13948 NM_001913 NP_001904.2


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Images

Immunohistochemistry

Anti-CUX1 / CUTL1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.
Anti-CUX1 / CUTL1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.

Immunohistochemistry

Anti-CUX1 / CUTL1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.
Anti-CUX1 / CUTL1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CUTL1 on HeLa cell (antibody concentration 10 ug/ml).
Immunofluorescence of monoclonal antibody to CUTL1 on HeLa cell (antibody concentration 10 ug/ml).

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CUTL1 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CUTL1 is approximately 0.03 ng/ml as a capture antibody.

Western blot

Western blot of CUX1 expression in transfected 293T cell line by CUTL1 monoclonal antibody, clone 2A10. Lane 1: Transfected cell lysate Lane 2: Non-transfected lysate.
Western blot of CUX1 expression in transfected 293T cell line by CUTL1 monoclonal antibody, clone 2A10. Lane 1: Transfected cell lysate Lane 2: Non-transfected lysate.

Immunohistochemistry

Anti-CUX1 / CUTL1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.
Anti-CUX1 / CUTL1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.

Immunohistochemistry

Anti-CUX1 / CUTL1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.
Anti-CUX1 / CUTL1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CUTL1 on HeLa cell (antibody concentration 10 ug/ml).
Immunofluorescence of monoclonal antibody to CUTL1 on HeLa cell (antibody concentration 10 ug/ml).

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CUTL1 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CUTL1 is approximately 0.03 ng/ml as a capture antibody.

Western blot

Western blot of CUX1 expression in transfected 293T cell line by CUTL1 monoclonal antibody, clone 2A10. Lane 1: Transfected cell lysate Lane 2: Non-transfected lysate.
Western blot of CUX1 expression in transfected 293T cell line by CUTL1 monoclonal antibody, clone 2A10. Lane 1: Transfected cell lysate Lane 2: Non-transfected lysate.

Immunohistochemistry

Anti-CUX1 / CUTL1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.
Anti-CUX1 / CUTL1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.

Immunohistochemistry

Anti-CUX1 / CUTL1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.
Anti-CUX1 / CUTL1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CUTL1 on HeLa cell (antibody concentration 10 ug/ml).
Immunofluorescence of monoclonal antibody to CUTL1 on HeLa cell (antibody concentration 10 ug/ml).

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CUTL1 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CUTL1 is approximately 0.03 ng/ml as a capture antibody.

Western blot

Western blot of CUX1 expression in transfected 293T cell line by CUTL1 monoclonal antibody, clone 2A10. Lane 1: Transfected cell lysate Lane 2: Non-transfected lysate.
Western blot of CUX1 expression in transfected 293T cell line by CUTL1 monoclonal antibody, clone 2A10. Lane 1: Transfected cell lysate Lane 2: Non-transfected lysate.

Immunohistochemistry

Anti-CUX1 / CUTL1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.
Anti-CUX1 / CUTL1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.

Immunohistochemistry

Anti-CUX1 / CUTL1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.
Anti-CUX1 / CUTL1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4309 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CUTL1 on HeLa cell (antibody concentration 10 ug/ml).
Immunofluorescence of monoclonal antibody to CUTL1 on HeLa cell (antibody concentration 10 ug/ml).

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CUTL1 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CUTL1 is approximately 0.03 ng/ml as a capture antibody.

Western blot

Western blot of CUX1 expression in transfected 293T cell line by CUTL1 monoclonal antibody, clone 2A10. Lane 1: Transfected cell lysate Lane 2: Non-transfected lysate.
Western blot of CUX1 expression in transfected 293T cell line by CUTL1 monoclonal antibody, clone 2A10. Lane 1: Transfected cell lysate Lane 2: Non-transfected lysate.

Requested From: United States
Date Requested: 10/21/2018

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