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Antibodies > CTTN / Cortactin > LS-C801185
 
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Catalog Number Size Price
LS-C801185-100 100 µl (1 mg/ml) $379 
LS-C801185-200 200 µl (1 mg/ml) $421 
CTTN / Cortactin Antibody - 1:200 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CTTN / Cortactin Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CTTN / Cortactin Antibody - Western blot analysis of Cortactin phosphorylation expression in COS7 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CTTN / Cortactin Antibody - Western blot analysis of Phospho-Cortactin (Tyr466) expression in various lysates
CTTN / Cortactin Antibody - 1:200 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CTTN / Cortactin Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CTTN / Cortactin Antibody - Western blot analysis of Cortactin phosphorylation expression in COS7 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CTTN / Cortactin Antibody - Western blot analysis of Phospho-Cortactin (Tyr466) expression in various lysates
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Polyclonal Rabbit anti‑Human CTTN / Cortactin Antibody (phospho‑Tyr466, IHC, IF, WB) LS‑C801185

Polyclonal Rabbit anti‑Human CTTN / Cortactin Antibody (phospho‑Tyr466, IHC, IF, WB) LS‑C801185

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Antibody:
CTTN / Cortactin Rabbit anti-Human Polyclonal (pTyr466) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801185-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CTTN / Cortactin Rabbit anti-Human Polyclonal (pTyr466) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Cortactin antibody LS-C801185 is an unconjugated rabbit polyclonal antibody to Cortactin (CTTN) (pTyr466) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human CTTN / Cortactin
Synonyms
CTTN | Cortactin | EMS1 | Oncogene EMS1 | Amplaxin | Src substrate cortactin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Cortactin around the phosphorylation site of Tyrosine 466.
Epitope
pTyr466
Specificity
Phospho-Cortactin (Tyr466) Antibody detects endogenous levels of Cortactin only when phosphorylated at Tyrosine 466.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CTTN / Cortactin
Q14247 NM_005231 NP_005222.2

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Featured Products

CTTN / Cortactin Antibody - IHC of p-Cortactin (Y421) pAb in paraffin-embedded human prostate carcinoma tissue.
CTTN / Cortactin Antibody (phospho‑Tyr421) LS‑C178045
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
CTTN / Cortactin Antibody - IHC of paraffin-embedded cervical cancer tissues (left) and tonsil tissues (right) using CTTN mouse monoclonal antibody with DAB staining.
CTTN / Cortactin Antibody (clone 4C6) LS‑C169338
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
CTTN / Cortactin Antibody - Immunohistochemical analysis of Cortactin (pY421) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CTTN / Cortactin Antibody (phospho‑Tyr421) LS‑C353975
Species: Human, Mouse
Applications: IHC - Paraffin, Western blot
CTTN / Cortactin Antibody - Western blot of Acetyl-Cortactin (K235) antibody
CTTN / Cortactin Antibody (Internal) LS‑C387609
Species: Human, Mouse, Rat
Applications: Western blot
CTTN / Cortactin Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.
IHC‑plus™ CTTN / Cortactin Antibody (aa450‑500) LS‑B15306
Species: Human
Applications: IHC, IHC - Paraffin
CTTN / Cortactin Antibody - Immunohistochemical analysis of Cortactin (pY466) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CTTN / Cortactin Antibody (phospho‑Tyr466) LS‑C353976
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
See all CTTN / Cortactin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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