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Catalog Number	Size	Price
LS-C343999-10	10 µg	$318
LS-C343999-100	100 µg (0.5 mg/ml)	$470

CTNNA1 / Catenin Alpha-1 Antibody - CTNNA1 antibody IHC-paraffin: Mouse Intestine Tissue.

CTNNA1 / Catenin Alpha-1 Antibody - CTNNA1 antibody IHC-paraffin: Human Mammary Tissue.

CTNNA1 / Catenin Alpha-1 Antibody - CTNNA1 antibody IHC-paraffin: Rat Intestine Tissue.

CTNNA1 / Catenin Alpha-1 Antibody - Western blot analysis of CTNNA1 using anti-CTNNA1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate Lane 2: Rat Lung Tissue Lysate Lane 3: Rat Cardiac Muscle Tissue Lysate Lane 4: NIH3T3 Whole Cell Lysate Lane 5: PC-12 Whole Cell Lysate Lane 6: HEPG2 Whole Cell Lysate Lane 7: HELA Whole Cell Lysate Lane 8: MCF-7 Whole Cell Lysate Lane 9: HEPA Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNA1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTNNA1 at approximately 100KD. The expected band size for CTNNA1 is at 100KD.

CTNNA1 / Catenin Alpha-1 Antibody - CTNNA1 antibody Western blot. All lanes: Anti CTNNA1 at 0.5 ug/ml. WB: Human Recombinant CTNNA1 Protein 0.5ng. Predicted band size: 47 kD. Observed band size: 47 kD.

CTNNA1 / Catenin Alpha-1 Antibody - Flow Cytometry analysis of U-87 cells using anti-CTNNA1 antibody. Overlay histogram showing U-87 cells stained with anti-CTNNA1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTNNA1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human CTNNA1 / Catenin Alpha‑1 Antibody (aa143‑292, IHC, WB) LS‑C343999

Name: Anti-CTNNA1 / Catenin Alpha-1 Antibody | Rabbit anti-Human IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C343999-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

CTNNA1 / Catenin Alpha-1 Rabbit anti-Human Polyclonal (aa143-292) Antibody

Application:

IHC, IHC-P, IHC-Fr, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C343999-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CTNNA1 / Catenin Alpha-1 Rabbit anti-Human Polyclonal (aa143-292) Antibody

Application:

IHC, IHC-P, IHC-Fr, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

Catenin Alpha-1 antibody LS-C343999 is an unconjugated rabbit polyclonal antibody to Catenin Alpha-1 (CTNNA1) (aa143-292) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.

Target

Human CTNNA1 / Catenin Alpha-1

Synonyms

See All CTNNA1 / Catenin Alpha-1 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human CTNNA1 recombinant protein (Position: D143-D292). Human CTNNA1 shares 98% amino acid (aa) sequence identity with mouse CTNNA1.

Epitope

aa143-292

Specificity

Expressed ubiquitously in normal tissues.

Applications

IHC
IHC - Paraffin
IHC - Frozen
Western blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CTNNA1 / Catenin Alpha-1

Uniprot: P35221 NCBI: NM_001903 NP_001894.2