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Catalog Number Size Price
LS-C312711-10 10 µg $318 
LS-C312711-100 100 µg (0.5 mg/ml) $470 
CTBP2 Antibody - IHC analysis of CTBP2 using anti-CTBP2 antibody. CTBP2 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CTBP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CTBP2 Antibody - IHC analysis of CTBP2 using anti-CTBP2 antibody. CTBP2 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CTBP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CTBP2 Antibody - IHC analysis of CTBP2 using anti-CTBP2 antibody. CTBP2 was detected in frozen section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CTBP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CTBP2 Antibody - IHC analysis of CTBP2 using anti-CTBP2 antibody. CTBP2 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CTBP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CTBP2 Antibody - CTBP2 antibody. IHC(P):Human Breast Cancer Tissue.
CTBP2 Antibody - CTBP2 antibody. ICC:HELA Cell.
CTBP2 Antibody - CTBP2 antibody. IHC(F): Rat Intestine Tissue.
CTBP2 Antibody - Western blot analysis of CTBP2 using anti-CTBP2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: HELA whole cell lysates, Lane 4: 293T whole cell lysates. Lane 5: Colo-320 whole cell lysates, Lane 6: U87 whole cell lysates, Lane 7: SW620 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTBP2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTBP2 at approximately 49KD. The expected band size for CTBP2 is at 49KD.
CTBP2 Antibody - WB of CTBP2 antibody. All lanes: Anti-CTBP2 at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 40ug. Lane 2: Rat Spleen Tissue Lysate at 40ug. Lane 3: HELA Whole Cell Lysate at 40ug. Lane 4: 293T Whole Cell Lysate at 40ug. Lane 5: COLO320 Whole Cell Lysate at 40ug. Lane 6: U87 Whole Cell Lysate at 40ug. Lane 7: SW620 Whole Cell Lysate at 40ug. Predicted bind size: 49KD. Observed bind size: 49KD.
CTBP2 Antibody - IHC analysis of CTBP2 using anti-CTBP2 antibody. CTBP2 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CTBP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CTBP2 Antibody - IHC analysis of CTBP2 using anti-CTBP2 antibody. CTBP2 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CTBP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CTBP2 Antibody - IHC analysis of CTBP2 using anti-CTBP2 antibody. CTBP2 was detected in frozen section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CTBP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CTBP2 Antibody - IHC analysis of CTBP2 using anti-CTBP2 antibody. CTBP2 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CTBP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CTBP2 Antibody - CTBP2 antibody. IHC(P):Human Breast Cancer Tissue.
CTBP2 Antibody - CTBP2 antibody. ICC:HELA Cell.
CTBP2 Antibody - CTBP2 antibody. IHC(F): Rat Intestine Tissue.
CTBP2 Antibody - Western blot analysis of CTBP2 using anti-CTBP2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: HELA whole cell lysates, Lane 4: 293T whole cell lysates. Lane 5: Colo-320 whole cell lysates, Lane 6: U87 whole cell lysates, Lane 7: SW620 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTBP2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTBP2 at approximately 49KD. The expected band size for CTBP2 is at 49KD.
CTBP2 Antibody - WB of CTBP2 antibody. All lanes: Anti-CTBP2 at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 40ug. Lane 2: Rat Spleen Tissue Lysate at 40ug. Lane 3: HELA Whole Cell Lysate at 40ug. Lane 4: 293T Whole Cell Lysate at 40ug. Lane 5: COLO320 Whole Cell Lysate at 40ug. Lane 6: U87 Whole Cell Lysate at 40ug. Lane 7: SW620 Whole Cell Lysate at 40ug. Predicted bind size: 49KD. Observed bind size: 49KD.
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Polyclonal Rabbit anti‑Human CTBP2 Antibody (aa429‑445, IHC, WB) LS‑C312711

Polyclonal Rabbit anti‑Human CTBP2 Antibody (aa429‑445, IHC, WB) LS‑C312711

Note: This antibody replaces LS-C388295
Antibody:
CTBP2 Rabbit anti-Human Polyclonal (aa429-445) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Goat, Guinea pig, Horse, Pig, Rabbit, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312711-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CTBP2 Rabbit anti-Human Polyclonal (aa429-445) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Goat, Guinea pig, Horse, Pig, Rabbit, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
CTBP2 antibody LS-C312711 is an unconjugated rabbit polyclonal antibody to CTBP2 (aa429-445) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IHC and WB.
Target
Human CTBP2
Synonyms
CTBP2 | C-terminal-binding protein 2 | C-terminal binding protein 2 | Ribeye
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Goat, Guinea pig, Horse, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human CTBP2(429-445 aa PNQPTKHGDNREHPNEQ), identical to the related rat and mouse sequences.
Epitope
aa429-445
Specificity
Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CTBP2
P56545 NM_022802 NP_073713.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024