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Catalog Number Size Price
LS-C407639-10 10 µg $318 
LS-C407639-100 100 µg $470 
CTBP1 / CTBP Antibody - CTBP1 antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
CTBP1 / CTBP Antibody - CTBP1 antibody IHC-paraffin. IHC(P): Rat Spleen Tissue.
CTBP1 / CTBP Antibody - CTBP1 antibody IHC-paraffin. IHC(P): Mouse Thymus Tissue.
CTBP1 / CTBP Antibody - IF analysis of CTBP1 using anti-CTBP1 antibody CTBP1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CTBP1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CTBP1 / CTBP Antibody - IF analysis of CTBP1 using anti-CTBP1 antibody CTBP1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CTBP1 Antibody overnight at 4°C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CTBP1 / CTBP Antibody - IF analysis of CTBP1 using anti-CTBP1 antibody CTBP1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CTBP1 Antibody overnight at 4°C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CTBP1 / CTBP Antibody - CTBP1 antibody Western blot. All lanes: Anti CTBP1 at 0.5 ug/ml. Lane 1: Rat Lung Tissue Lysate at 50 ug. Lane 2: Rat Kidney Tissue Lysate at 50 ug. Lane 3: COLO320 Whole Cell Lysate at 40 ug. Lane 4: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 48 kD. Observed band size: 48 kD.
CTBP1 / CTBP Antibody - Flow Cytometry analysis of U20S cells using anti-CTBP1 antibody. Overlay histogram showing U20S cells stained with anti-CTBP1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTBP1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CTBP1 / CTBP Antibody - CTBP1 antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
CTBP1 / CTBP Antibody - CTBP1 antibody IHC-paraffin. IHC(P): Rat Spleen Tissue.
CTBP1 / CTBP Antibody - CTBP1 antibody IHC-paraffin. IHC(P): Mouse Thymus Tissue.
CTBP1 / CTBP Antibody - IF analysis of CTBP1 using anti-CTBP1 antibody CTBP1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CTBP1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CTBP1 / CTBP Antibody - IF analysis of CTBP1 using anti-CTBP1 antibody CTBP1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CTBP1 Antibody overnight at 4°C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CTBP1 / CTBP Antibody - IF analysis of CTBP1 using anti-CTBP1 antibody CTBP1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CTBP1 Antibody overnight at 4°C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CTBP1 / CTBP Antibody - CTBP1 antibody Western blot. All lanes: Anti CTBP1 at 0.5 ug/ml. Lane 1: Rat Lung Tissue Lysate at 50 ug. Lane 2: Rat Kidney Tissue Lysate at 50 ug. Lane 3: COLO320 Whole Cell Lysate at 40 ug. Lane 4: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 48 kD. Observed band size: 48 kD.
CTBP1 / CTBP Antibody - Flow Cytometry analysis of U20S cells using anti-CTBP1 antibody. Overlay histogram showing U20S cells stained with anti-CTBP1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTBP1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human CTBP1 / CTBP Antibody (aa409‑440, IHC, WB) LS‑C407639

Polyclonal Rabbit anti‑Human CTBP1 / CTBP Antibody (aa409‑440, IHC, WB) LS‑C407639

Antibody:
CTBP1 / CTBP Rabbit anti-Human Polyclonal (aa409-440) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407639-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CTBP1 / CTBP Rabbit anti-Human Polyclonal (aa409-440) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CTBP antibody LS-C407639 is an unconjugated rabbit polyclonal antibody to CTBP (CTBP1) (aa409-440) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human CTBP1 / CTBP
Synonyms
CTBP1 | BARS | C-terminal binding protein 1 | C-terminal-binding protein 1 | CTBP
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human CtBP1 (409-440 aa HGLPPVAHPPHAPSPGQTVKPEADRDHASDQL), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
Epitope
aa409-440
Specificity
Expressed in germinal center B-cells. .
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CTBP1 / CTBP
Q13363 NM_001328 NP_001319.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024