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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CSTF2 / CstF‑64 Antibody (aa527‑577) LS‑C286468
CstF-64 antibody LS-C286468 is an unconjugated rabbit polyclonal antibody to CstF-64 (CSTF2) from human, mouse, rat and other species. Validated for IF and IHC.
Catalog
Size
Price
LS-C286468-100
100 µl
$415

Popular CSTF2 / CstF-64 Products

Immunoperoxidase of monoclonal antibody to CSTF2 on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 3 ug/ml].
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Immunohistochemical analysis of CstF-64 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey
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Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Monkey, Rat, Bovine, Dog, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Western blot of CstF-64 antibody
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

CstF-64 antibody LS-C286468 is an unconjugated rabbit polyclonal antibody to CstF-64 (CSTF2) from human, mouse, rat and other species. Validated for IF and IHC.
About CSTF2 / CstF-64
One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs. P33240 NM_001325 NP_001316.1

CSTF2 Antibody, BetaCstF-64 variant 2 Antibody, CstF-64 Antibody, CSTF 64 kDa subunit Antibody, CF-1 64 kDa subunit Antibody


Specifications

Target
Human CSTF2 / CstF-64
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:500)
  • Immunofluorescence (1:50 - 1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
aa527-577
Specificity
Region between residue 527 and 577 of human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kD using the numbering given in entry NP_001316.1 (GeneID 1478).
Usage
Immunohistochemistry: Antigen retrieval is recommended. Antigen retrieval with citrate buffer will enhance staining. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated. Human controls: Breast Carcinoma, Colon Carcinoma, Ewing Sarcoma, Linitis Plastica Stomach Cancer, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Skin Basal Cell Carcinoma, Small Cell Lung Cancer, Stomach Adenocarcinoma, Testicular Seminoma. Mouse controls: Teratoma.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
TBS, 0.09% Sodium Azide, 0.1% BSA
Storage
Store at 2°C to 8°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Detection of Mouse CSTF64 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.
Detection of Mouse CSTF64 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.

Immunohistochemistry

Detection of Human CSTF64 by Immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.
Detection of Human CSTF64 by Immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.

Immunohistochemistry

Detection of Mouse CSTF64 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.
Detection of Mouse CSTF64 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.

Immunohistochemistry

Detection of Human CSTF64 by Immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.
Detection of Human CSTF64 by Immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.

Immunohistochemistry

Detection of Mouse CSTF64 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.
Detection of Mouse CSTF64 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.

Immunohistochemistry

Detection of Human CSTF64 by Immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.
Detection of Human CSTF64 by Immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.

Immunohistochemistry

Detection of Mouse CSTF64 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.
Detection of Mouse CSTF64 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.

Immunohistochemistry

Detection of Human CSTF64 by Immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.
Detection of Human CSTF64 by Immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:250.

Requested From: United States
Date Requested: 8/15/2018

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