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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CSTF1 Antibody LS‑C155256
CSTF1 antibody LS-C155256 is an unconjugated rabbit polyclonal antibody to human CSTF1. Validated for WB.
Catalog
Size
Price
LS-C155256-100
100 µl (1 mg/ml)
Unavailable

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Immunohistochemical analysis of CstF-50 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Product Description

CSTF1 antibody LS-C155256 is an unconjugated rabbit polyclonal antibody to human CSTF1. Validated for WB.
About CSTF1
CSTF1 is one of three (including CSTF2 and CSTF3) cleavage stimulation factors which combine to form CSTF which is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, CSTF1, contains transducin-like repeats. Q05048 NM_001324 NP_001315.1

CSTF1 Antibody, CstF-50 Antibody, CstFp50 Antibody, CF-1 50 kDa subunit Antibody, CSTF 50 kDa subunit Antibody


Specifications

Target
Human CSTF1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:3000)
Immunogen
CSTF1 antibody was raised against recombinant fragment corresponding to a region within amino acids 185 and 408 of cleavage stimulation factor (SwissProt Q05048). Percent identity by BLAST analysis: Human (100%); Porcine, Bovine (99%); Mouse, Rat (98%); Xenopus (92%).
Specificity
Human CSTF1
Usage
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Presentation
PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Storage
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE CSTF1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE CSTF1 antibody diluted at 1:1000

Western blot

Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE CSTF1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE CSTF1 antibody diluted at 1:1000

Western blot

Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE CSTF1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE CSTF1 antibody diluted at 1:1000

Western blot

Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE CSTF1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE CSTF1 antibody diluted at 1:1000


Requested From: 
Date Requested: 6/23/2018

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