Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Mouse, Rat, Bovine, Xenopus (at least 90% immunogen sequence identity)
Western blot (1:500 - 1:3000)
CSTF1 antibody was raised against recombinant fragment corresponding to a region within amino acids 185 and 408 of cleavage stimulation factor (SwissProt Q05048). Percent identity by BLAST analysis: Human (100%); Porcine, Bovine (99%); Mouse, Rat (98%); Xenopus (92%).
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
CSTF1 is one of three (including CSTF2 and CSTF3) cleavage stimulation factors which combine to form CSTF which is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, CSTF1, contains transducin-like repeats.