G-CSF antibody LS-C15557 is an unconjugated rat monoclonal antibody to human G-CSF (CSF3). Validated for ELISA and WB.
Human CSF3 / G-CSF
(tested or 100% immunogen sequence identity)
CSF3 / G-CSF antibody was raised against e. coli-expressed recombinant human G-CSF.
Recognizes human Granulocyte-Colony Stimulating Factor (G-CSF).
ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying human G-CSF protein levels.1, 2 Purified antibody should be paired with biotinylated antibody (Cat. Western Blot: Useful for Western blot applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human G-CSF under reducing conditions.
100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.