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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CSA / ERCC8 Antibody IHC‑plus™ LS‑B4076
Note: This antibody replaces LS-C109291
ERCC8 antibody LS-B4076 is an unconjugated rabbit polyclonal antibody to ERCC8 (CSA) from human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4076-50
50 µl
Unavailable

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Product Description

ERCC8 antibody LS-B4076 is an unconjugated rabbit polyclonal antibody to ERCC8 (CSA) from human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CSA / ERCC8
Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. Q13216 NM_000082 NP_000073.1

ERCC8 Antibody, CKN1 Antibody, UVSS2 Antibody, CSA Antibody


Specifications

Target
Human CSA / ERCC8
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ICC
  • Immunofluorescence (1:100 - 1:200)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CSA / ERCC8 antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 106 and 300 of CSA.
Specificity
Human ERCC8.
Usage
Cross reactivity with mouse has been confirmed in WB only. The suitability of the antibody for use in IHC with mouse samples has not been evaluated at this time. Cross-reactivity with rat and cow has not been experimentally confirmed. The antibody is predicted to cross-react with rat and cow based on sequence homologies of 96% and 95% respectively.
Presentation
PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-ERCC8 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4076 concentration 10 ug/ml.
Anti-ERCC8 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4076 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence of paraformaldehyde-fixed A431, using ERCC8(CSA) antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431, using ERCC8(CSA) antibody at 1:200 dilution.

Western blot

Sample(30 g of whole cell lysate). A:293T. B: A431. 12% SDS PAGE. ERCC8 antibody diluted at 1:500.
Sample(30 g of whole cell lysate). A:293T. B: A431. 12% SDS PAGE. ERCC8 antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. ERCC8 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. ERCC8 antibody diluted at 1:1000.

Immunohistochemistry

Anti-ERCC8 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4076 concentration 10 ug/ml.
Anti-ERCC8 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4076 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence of paraformaldehyde-fixed A431, using ERCC8(CSA) antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431, using ERCC8(CSA) antibody at 1:200 dilution.

Western blot

Sample(30 g of whole cell lysate). A:293T. B: A431. 12% SDS PAGE. ERCC8 antibody diluted at 1:500.
Sample(30 g of whole cell lysate). A:293T. B: A431. 12% SDS PAGE. ERCC8 antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. ERCC8 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. ERCC8 antibody diluted at 1:1000.

Immunohistochemistry

Anti-ERCC8 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4076 concentration 10 ug/ml.
Anti-ERCC8 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4076 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence of paraformaldehyde-fixed A431, using ERCC8(CSA) antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431, using ERCC8(CSA) antibody at 1:200 dilution.

Western blot

Sample(30 g of whole cell lysate). A:293T. B: A431. 12% SDS PAGE. ERCC8 antibody diluted at 1:500.
Sample(30 g of whole cell lysate). A:293T. B: A431. 12% SDS PAGE. ERCC8 antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. ERCC8 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. ERCC8 antibody diluted at 1:1000.

Immunohistochemistry

Anti-ERCC8 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4076 concentration 10 ug/ml.
Anti-ERCC8 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4076 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence of paraformaldehyde-fixed A431, using ERCC8(CSA) antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431, using ERCC8(CSA) antibody at 1:200 dilution.

Western blot

Sample(30 g of whole cell lysate). A:293T. B: A431. 12% SDS PAGE. ERCC8 antibody diluted at 1:500.
Sample(30 g of whole cell lysate). A:293T. B: A431. 12% SDS PAGE. ERCC8 antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. ERCC8 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. ERCC8 antibody diluted at 1:1000.


Requested From: 
Date Requested: 6/21/2018

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