ERCC8 antibody LS-C112132 is an unconjugated rabbit polyclonal antibody to ERCC8 (CSA) from human, rat, dog and other species. Validated for WB.
Human, Rat, Dog, Hamster, Horse, Pig
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
CSA / ERCC8 antibody was raised against synthetic peptide located between aa216-265 of human ERCC8 (Q13216, NP_000073). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Rat, Hamster, Elephant, Dog, Horse, Pig (100%); Galago, Marmoset, Mouse, Bovine, Bat, Rabbit (92%); Panda, Guinea pig (85%).
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.