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Catalog Number Size Price
LS-C369473-50 50 µg $294 
LS-C369473-100 100 µg $360 
CRYAB / Alpha B Crystallin Antibody - Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CRYAB / Alpha B Crystallin Antibody - Immunohistochemistry of paraffin-embedded human kidney tissue using CRYAB Antibody at dilution of 1:100
CRYAB / Alpha B Crystallin Antibody - IHC image of CRYAB Antibody diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CRYAB / Alpha B Crystallin Antibody - Immunofluorescence staining of U251 cells with CRYAB Antibody at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
CRYAB / Alpha B Crystallin Antibody - Western blot All lanes: Alpha-crystallin B chain antibody at 2µg/ml Lane 1: 293T whole cell lysate Lane 2: Mouse eye tissue Lane 3: Rat lung tissue Lane 4: Rat heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa
CRYAB / Alpha B Crystallin Antibody - Western blot. All lanes: Alpha-crystallin B chain antibody at 2 ug/ml. Lane 1: 293T whole cell lysate Lane 2: mouse eye tissue Lane 3: rat lung tissue Lane 3: rat heart tissue. Secondary antibody: goat polyclonal to rabbit at 1:10000 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CRYAB / Alpha B Crystallin Antibody - Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CRYAB / Alpha B Crystallin Antibody - Immunohistochemistry of paraffin-embedded human kidney tissue using CRYAB Antibody at dilution of 1:100
CRYAB / Alpha B Crystallin Antibody - IHC image of CRYAB Antibody diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CRYAB / Alpha B Crystallin Antibody - Immunofluorescence staining of U251 cells with CRYAB Antibody at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
CRYAB / Alpha B Crystallin Antibody - Western blot All lanes: Alpha-crystallin B chain antibody at 2µg/ml Lane 1: 293T whole cell lysate Lane 2: Mouse eye tissue Lane 3: Rat lung tissue Lane 4: Rat heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa
CRYAB / Alpha B Crystallin Antibody - Western blot. All lanes: Alpha-crystallin B chain antibody at 2 ug/ml. Lane 1: 293T whole cell lysate Lane 2: mouse eye tissue Lane 3: rat lung tissue Lane 3: rat heart tissue. Secondary antibody: goat polyclonal to rabbit at 1:10000 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Polyclonal Rabbit anti‑Human CRYAB / Alpha B Crystallin Antibody (aa1‑175, IHC, WB) LS‑C369473

Polyclonal Rabbit anti‑Human CRYAB / Alpha B Crystallin Antibody (aa1‑175, IHC, WB) LS‑C369473

Antibody:
CRYAB / Alpha B Crystallin Rabbit anti-Human Polyclonal (aa1-175) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$294
LS-C369473-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CRYAB / Alpha B Crystallin Rabbit anti-Human Polyclonal (aa1-175) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Alpha B Crystallin antibody LS-C369473 is an unconjugated rabbit polyclonal antibody to Alpha B Crystallin (CRYAB) (aa1-175) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Target
Human CRYAB / Alpha B Crystallin
Synonyms
CRYAB | Alpha-crystallin B chain | CRYA2 | Crystallin, alpha B | CTPP2 | Heat shock protein beta-5 | HSPB5 | Alpha B Crystallin | Alpha(B)-crystallin | Rosenthal fiber component | Heat-shock 20 kD like-protein
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, FITC, Biotin.
Purification
Caprylic acid and ammonium sulfate precipitation
Modifications
Unmodified
Immunogen
Recombinant human Alpha-crystallin B chain protein (aa1-175).
Epitope
aa1-175
Specificity
Human CRYAB / Alpha B Crystallin
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CRYAB / Alpha B Crystallin
P02511 NM_001885 NP_001876.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/6/2024