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Antibodies > CRKL > LS-C761160
 
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Catalog Number Size Price
LS-C761160-30 30 µl (1 mg/ml) $255 
LS-C761160-100 100 µl (1 mg/ml) $299 
LS-C761160-200 200 µl (1 mg/ml) $351 
CRKL Antibody - Immunohistochemical analysis of CRKL staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CRKL Antibody - Immunofluorescent analysis of CRKL staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
CRKL Antibody - Western blot analysis of CRKL expression in HEK293T (A), SGC7901 (B) whole cell lysates.
CRKL Antibody - Immunohistochemical analysis of CRKL staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CRKL Antibody - Immunofluorescent analysis of CRKL staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
CRKL Antibody - Western blot analysis of CRKL expression in HEK293T (A), SGC7901 (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human CRKL Antibody (IHC, IF, WB) LS‑C761160

Polyclonal Rabbit anti‑Human CRKL Antibody (IHC, IF, WB) LS‑C761160

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Antibody:
CRKL Rabbit anti-Human Polyclonal Antibody
Application:
IHC, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255
LS-C761160-30
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CRKL Rabbit anti-Human Polyclonal Antibody
Application:
IHC, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CRKL antibody LS-C761160 is an unconjugated rabbit polyclonal antibody to CRKL from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human CRKL
Synonyms
CRKL | Crk-like protein
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CRKL. The exact sequence is proprietary.
Specificity
Recognizes endogenous levels of CRKL protein.
Applications
  • IHC (1:50 - 1:100)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.42% Potassium Phosphate, pH 7.3, 0.87% NaCl, 0.01% Sodium Azide, 30% Glycerol
Storage
Upon delivery, aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CRKL
P46109 NM_005207 NP_005198.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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