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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CRKL Antibody (aa183‑232, HRP) LS‑C425912
CRKL antibody LS-C425912 is an HRP-conjugated rabbit polyclonal antibody to CRKL from human and chimpanzee. Validated for IHC and WB.
Catalog
Size
Price
LS-C425912-100
100 µl (0.65 mg/ml)
Unavailable

Popular CRKL Products

Antibody
Species: Human
Applications: Western blot
IHC of paraffin-embedded human lung carcinoma tissue, using CrkL (Ab-207) Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-CRKL antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6441 concentration 2.5 ug/ml.
Species: Human, Monkey, Bat, Chicken
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunofluorescence of monoclonal antibody to CRKL on HeLa cell . [antibody concentration 10 ug/ml]
Species: Human
Applications: Immunofluorescence, Western blot, ELISA, RNA interference
IHC of paraffin-embedded human lung carcinoma, using CrkL (Phospho-Tyr207) Antibody. The sample on the right was incubated with synthetic peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

CRKL antibody LS-C425912 is an HRP-conjugated rabbit polyclonal antibody to CRKL from human and chimpanzee. Validated for IHC and WB.
About CRKL
CRKL is a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic. P46109 NM_005207 NP_005198.1

CRKL Antibody, Crk-like protein Antibody


Specifications

Target
Human CRKL
Host
Rabbit
Reactivity
Human, Chimpanzee (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with Biotin, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CRKL antibody was raised against synthetic peptide located between aa183-232 of human CRKL (P46109, NP_005198). Percent identity by BLAST analysis: Human, Chimpanzee (100%).
Epitope
aa183-232
Specificity
Human CRKL
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
100 mM sodium phosphate, pH 7.6, 150 mM NaCl
Storage
Short term, 4°C. Long term, add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

CRKL antibody Immunohistochemistry of Human Lung cell. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CRKL antibody Immunohistochemistry of Human Lung cell. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CRKL antibody Western Blot of K562.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CRKL antibody Western Blot of K562. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

CRKL antibody Immunohistochemistry of Human Lung cell. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CRKL antibody Immunohistochemistry of Human Lung cell. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CRKL antibody Western Blot of K562.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CRKL antibody Western Blot of K562. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

CRKL antibody Immunohistochemistry of Human Lung cell. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CRKL antibody Immunohistochemistry of Human Lung cell. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CRKL antibody Western Blot of K562.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CRKL antibody Western Blot of K562. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

CRKL antibody Immunohistochemistry of Human Lung cell. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CRKL antibody Immunohistochemistry of Human Lung cell. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CRKL antibody Western Blot of K562.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CRKL antibody Western Blot of K562. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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