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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CPOX Antibody (aa139‑423) LS‑C185681
CPOX antibody LS-C185681 is an unconjugated rabbit polyclonal antibody to human CPOX. Validated for WB.
Catalog
Size
Price
LS-C185681-100
100 µl (0.42 mg/ml)
$335

Popular CPOX Products

ICC/IF analysis of CPOX in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CPOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Species: Human
Applications: ICC, Western blot, Flow Cytometry, ELISA
Immunofluorescence staining of HepG2 cells at a dilution of 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
Species: Human
Applications: Immunofluorescence, Western blot
Western blot of CPOX Antibody with human 721_B Whole Cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot
Western blot Image
Species: Human
Applications: Western blot

Product Description

CPOX Antibody (aa139-423) for WB/Western LS-C185681

Specifications

Target
Human CPOX
Synonyms
CPOX, Coproporphyrinogenase, Coproporphyrinogen iii oxidase, Coprogen oxidase, CPX, HCP, Coproporphyrinogen oxidase, COX
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Bovine (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:3000)
Immunogen
CPOX antibody was raised against recombinant fragment corresponding to a region within amino acids 139 and 423 of CPOX (SwissProt P36551). Percent identity by BLAST analysis: Human (100%); Bovine (94%); Mouse (89%); Rat (88%).
Epitope
aa139-423
Specificity
Human CPOX
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
About CPOX
Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX. P36551 NM_000097 NP_000088.3


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Images

Western blot

Sample (30 ug of whole cell lysate). A: HCT116 10% SDS PAGE. CPOX antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: HCT116 10% SDS PAGE. CPOX antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: HCT116 10% SDS PAGE. CPOX antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: HCT116 10% SDS PAGE. CPOX antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: HCT116 10% SDS PAGE. CPOX antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: HCT116 10% SDS PAGE. CPOX antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: HCT116 10% SDS PAGE. CPOX antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: HCT116 10% SDS PAGE. CPOX antibody diluted at 1:1000.

Requested From: United States
Date Requested: 10/22/2018

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