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Antibodies > COXIV / COX4 > LS-C662961
 
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Catalog Number Size Price
LS-C662961-10 10 µg $318 
LS-C662961-100 100 µg $470 
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody. COX IV was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-COX IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody. COX IV was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-COX IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - Western blot analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - Western blot analysis of COX IV using anti-COX IV antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates,Lane 2: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX IV antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COX IV at approximately 17KD. The expected band size for COX IV is at 20KD.
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - Western blot analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody. COX IV was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-COX IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody. COX IV was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-COX IV Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - Western blot analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - Western blot analysis of COX IV using anti-COX IV antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates,Lane 2: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX IV antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COX IV at approximately 17KD. The expected band size for COX IV is at 20KD.
COXIV / COX4 Antibody - IHC analysis of COX IV using anti-COX IV antibody
COXIV / COX4 Antibody - Western blot analysis of COX IV using anti-COX IV antibody
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Polyclonal Rabbit anti‑Human COXIV / COX4 Antibody (IHC, WB) LS‑C662961

Polyclonal Rabbit anti‑Human COXIV / COX4 Antibody (IHC, WB) LS‑C662961

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Antibody:
COXIV / COX4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662961-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
COXIV / COX4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
COX4 antibody LS-C662961 is an unconjugated rabbit polyclonal antibody to human COX4 (COXIV). Validated for ELISA, IHC and WB.
Target
Human COXIV / COX4
Synonyms
COX4I1 | COX4-1 | COX4 | COX IV-1 | COXIV
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived human COX IV recombinant protein (Position: Q59-K169).
Specificity
Ubiquitous.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About COXIV / COX4
P13073 NM_001861 NP_001852.1

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IHC‑plus™ COXIV / COX4 Antibody (aa154‑166) LS‑B3256
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
COXIV / COX4 Antibody - Anti-COXIV / COX4 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC‑plus™ COXIV / COX4 Antibody (clone 6B3) LS‑B10726
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
COXIV / COX4 Antibody - Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using COX41 Antibody. The picture on the right is blocked with the synthesized peptide.
COXIV / COX4 Antibody (aa11‑60) LS‑C119480
Species: Human
Applications: IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
COXIV / COX4 Antibody - Anti-COXIV / COX4 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
IHC‑plus™ COXIV / COX4 Antibody (aa1‑169) LS‑B10751
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
COXIV / COX4 Antibody - Immunofluorescent analysis of COX4-1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
COXIV / COX4 Antibody LS‑C413515
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot
COXIV / COX4 Antibody - COX4I1 antibody ARP42784_T100-NP_001852-COX4I1(cytochrome c oxidase subunit IV isoform 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.
COXIV / COX4 Antibody (N‑Terminus) LS‑C31848
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
See all COXIV / COX4 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
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