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Catalog Number Size Price
LS-C407703-10 10 µg $318 
LS-C407703-100 100 µg $470 
COPS5 / JAB1 Antibody - JAB1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
COPS5 / JAB1 Antibody - JAB1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.
COPS5 / JAB1 Antibody - JAB1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.
COPS5 / JAB1 Antibody - ICC analysis of JAB1 using anti-JAB1 antibody. JAB1 was detected in immunocytochemical section of A431 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-JAB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COPS5 / JAB1 Antibody - JAB1 antibody Western blot. All lanes: Anti JAB1 at 0.5 ug/ml. Lane 1: Rat Testis Tissue Lysate at 50 ug. Lane 2: Mouse Testis Tissue Lysate at 50 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Lane 4: 293T Whole Cell Lysate at 40 ug. Predicted band size: 38 kD. Observed band size: 38 kD.
COPS5 / JAB1 Antibody - Flow Cytometry analysis of U937 cells using anti-JAB1 antibody. Overlay histogram showing U937 cells stained with anti-JAB1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JAB1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
COPS5 / JAB1 Antibody - Flow Cytometry analysis of U20S cells using anti-JAB1 antibody. Overlay histogram showing U20S cells stained with anti-JAB1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JAB1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
COPS5 / JAB1 Antibody - JAB1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
COPS5 / JAB1 Antibody - JAB1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.
COPS5 / JAB1 Antibody - JAB1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.
COPS5 / JAB1 Antibody - ICC analysis of JAB1 using anti-JAB1 antibody. JAB1 was detected in immunocytochemical section of A431 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-JAB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COPS5 / JAB1 Antibody - JAB1 antibody Western blot. All lanes: Anti JAB1 at 0.5 ug/ml. Lane 1: Rat Testis Tissue Lysate at 50 ug. Lane 2: Mouse Testis Tissue Lysate at 50 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Lane 4: 293T Whole Cell Lysate at 40 ug. Predicted band size: 38 kD. Observed band size: 38 kD.
COPS5 / JAB1 Antibody - Flow Cytometry analysis of U937 cells using anti-JAB1 antibody. Overlay histogram showing U937 cells stained with anti-JAB1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JAB1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
COPS5 / JAB1 Antibody - Flow Cytometry analysis of U20S cells using anti-JAB1 antibody. Overlay histogram showing U20S cells stained with anti-JAB1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JAB1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human COPS5 / JAB1 Antibody (aa8‑334, IHC, WB) LS‑C407703

Polyclonal Rabbit anti‑Human COPS5 / JAB1 Antibody (aa8‑334, IHC, WB) LS‑C407703

Antibody:
COPS5 / JAB1 Rabbit anti-Human Polyclonal (aa8-334) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407703-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
COPS5 / JAB1 Rabbit anti-Human Polyclonal (aa8-334) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
JAB1 antibody LS-C407703 is an unconjugated rabbit polyclonal antibody to JAB1 (COPS5) (aa8-334) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human COPS5 / JAB1
Synonyms
COPS5 | 38 kDa Mov34 homolog | JAB1 | Signalosome subunit 5 | MOV-34 | SGN5 | CSN5
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human JAB1 recombinant protein (Position: M8-S334). Human JAB1 shares 99.4% amino acid (aa) sequence identity with mouse JAB1.
Epitope
aa8-334
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About COPS5 / JAB1
Q92905 NM_006837 NP_006828.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024