Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > COPE > LS-C662217
 
Print Page  
Catalog Number Size Price
LS-C662217-10 10 µg $318 
LS-C662217-100 100 µg $470 
COPE Antibody - IHC analysis of COPE using anti-COPE antibody. COPE was detected in paraffin-embedded section of rat pancreas tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-COPE Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COPE Antibody - IHC analysis of COPE using anti-COPE antibody. COPE was detected in paraffin-embedded section of mouse pancreas tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-COPE Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COPE Antibody - IHC analysis of COPE using anti-COPE antibody. COPE was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-COPE Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COPE Antibody - IF analysis of COPE using anti-COPE antibody COPE was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-COPE Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
COPE Antibody - Western blot analysis of COPE using anti-COPE antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat stomach tissue lysate,Lane 2: rat small intestine tissue lysate,Lane 3: rat pancreas tissue lysate,Lane 4: mouse stomach tissue lysate,Lane 5: mouse small intestine tissue lysate,Lane 6: mouse pancreas tissue lysate,Lane 7: human MCF-7 whole Cell lysatem,Lane 8: human Hela whole Cell lysate,Lane 9: human 22RV1 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COPE antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COPE at approximately 34KD. The expected band size for COPE is at 34KD.
COPE Antibody - Flow Cytometry analysis of HepG2 cells using anti-COPE antibody. Overlay histogram showing HepG2 cells stained with anti-COPE antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-COPE Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
COPE Antibody - IHC analysis of COPE using anti-COPE antibody. COPE was detected in paraffin-embedded section of rat pancreas tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-COPE Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COPE Antibody - IHC analysis of COPE using anti-COPE antibody. COPE was detected in paraffin-embedded section of mouse pancreas tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-COPE Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COPE Antibody - IHC analysis of COPE using anti-COPE antibody. COPE was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-COPE Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COPE Antibody - IF analysis of COPE using anti-COPE antibody COPE was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-COPE Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
COPE Antibody - Western blot analysis of COPE using anti-COPE antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat stomach tissue lysate,Lane 2: rat small intestine tissue lysate,Lane 3: rat pancreas tissue lysate,Lane 4: mouse stomach tissue lysate,Lane 5: mouse small intestine tissue lysate,Lane 6: mouse pancreas tissue lysate,Lane 7: human MCF-7 whole Cell lysatem,Lane 8: human Hela whole Cell lysate,Lane 9: human 22RV1 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COPE antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COPE at approximately 34KD. The expected band size for COPE is at 34KD.
COPE Antibody - Flow Cytometry analysis of HepG2 cells using anti-COPE antibody. Overlay histogram showing HepG2 cells stained with anti-COPE antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-COPE Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
1 of 6
2 of 6
3 of 6
4 of 6
5 of 6
6 of 6

Polyclonal Rabbit anti‑Human COPE Antibody (IHC, WB) LS‑C662217

Polyclonal Rabbit anti‑Human COPE Antibody (IHC, WB) LS‑C662217

Submit Review LSBio Guarantee
Antibody:
COPE Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662217-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
COPE Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
COPE antibody LS-C662217 is an unconjugated rabbit polyclonal antibody to human COPE. Validated for IHC and WB.
Target
Human COPE
Synonyms
COPE | Coatomer subunit epsilon | Coatomer epsilon subunit | Epsilon coat protein | Epsilon-coat protein | Epsilon-COP
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human COPE recombinant protein (Position: E80-A308). Human COPE shares 89.5% amino acid (aa) sequence identity with mouse COPE.
Applications
  • IHC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About COPE
O14579 NM_007263 NP_009194.2

Publications (0)

Submit a Publication for LS-C662217

Customer Reviews (0)

Submit a review for LS-C662217

Featured Products

Product
COPE Antibody (aa273‑300, APC) LS‑C213384
Species: Human
Applications: IHC, ICC, Immunofluorescence, Western blot, ELISA
Product
COPE Antibody (aa273‑300, Biotin) LS‑C213385
Species: Human
Applications: IHC, ICC, Immunofluorescence, Western blot, ELISA
Product
COPE Antibody (aa273‑300, FITC) LS‑C213386
Species: Human
Applications: IHC, ICC, Immunofluorescence, Western blot, ELISA
Product
COPE Antibody (aa273‑300, Azide‑free, HRP) LS‑C213387
Species: Human
Applications: IHC, ICC, Immunofluorescence, Western blot, ELISA
COPE Protein
Human COPE Protein (Recombinant His) - LS-G3085
Source: E. coli
Tag: His
COPE Protein - 12.5% SDS-PAGE of human COPE stained with Coomassie Blue
Human COPE Protein (Recombinant GST) (Full Length) - LS-G32605
Source: Wheat
Tag: GST
See all COPE Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/4/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com