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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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COPB2 / Beta‑COP Antibody LS‑C482752
Beta-COP antibody LS-C482752 is an unconjugated rabbit polyclonal antibody to Beta-COP (COPB2) from human, mouse and rat. Validated for ICC, IF and WB.
Catalog
Size
Price
LS-C482752-50
50 µl (1 mg/ml)
$255
LS-C482752-100
100 µl (1 mg/ml)
$295
LS-C482752-200
200 µl (1 mg/ml)
$375
Description
Beta-COP antibody LS-C482752 is an unconjugated rabbit polyclonal antibody to Beta-COP (COPB2) from human, mouse and rat. Validated for ICC, IF and WB.
Target
Human COPB2 / Beta-COP
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC (1:50 - 1:100)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:500 - 1:2000)
Immunogen
Recombinant full length human p102.
Specificity
Recognizes endogenous levels of p102 protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About COPB2 / Beta-COP
P35606 NM_004766 NP_004757.1

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Images

Immunofluorescence

Immunofluorescent analysis of p102 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunofluorescent analysis of p102 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot

Western blot analysis of p102 expression in HeLa (A); HepG2 (B); mouse liver (C); mouse kidney (D) whole cell lysates.
Western blot analysis of p102 expression in HeLa (A); HepG2 (B); mouse liver (C); mouse kidney (D) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of p102 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunofluorescent analysis of p102 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot

Western blot analysis of p102 expression in HeLa (A); HepG2 (B); mouse liver (C); mouse kidney (D) whole cell lysates.
Western blot analysis of p102 expression in HeLa (A); HepG2 (B); mouse liver (C); mouse kidney (D) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of p102 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunofluorescent analysis of p102 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot

Western blot analysis of p102 expression in HeLa (A); HepG2 (B); mouse liver (C); mouse kidney (D) whole cell lysates.
Western blot analysis of p102 expression in HeLa (A); HepG2 (B); mouse liver (C); mouse kidney (D) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of p102 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunofluorescent analysis of p102 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot

Western blot analysis of p102 expression in HeLa (A); HepG2 (B); mouse liver (C); mouse kidney (D) whole cell lysates.
Western blot analysis of p102 expression in HeLa (A); HepG2 (B); mouse liver (C); mouse kidney (D) whole cell lysates.

Requested From: United States
Date Requested: 12/13/2018
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