Complement C8b antibody LS-B5777 is an unconjugated rabbit polyclonal antibody to Complement C8b from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Human Complement C8b
Human, Monkey, Mouse, Rat, Dog, Guinea pig
(tested or 100% immunogen sequence identity)
Also available conjugated with FITC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
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Complement C8b antibody was raised against synthetic peptide located between aa451-500 of human C8B (P07358). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog (100%); Galago, Elephant, Bat, Rabbit, Horse, Pig (92%); Guinea pig (85%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5777. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.