Monkey Complement C3c

Goat

Monkey (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with Biotin, FITC, HRP.

Purified IgG

Unmodified

C3c purified from pooled normal rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

C3c fragment of Monkey complement factor C3.

• Immunofluorescence
• ELISA
• Dot Blot

In immunoelectrophoresis against fresh monkey serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other protein components of monkey serum or plasma. As unlabelled primary or secondary antibody reagent for the indirect detection of C3c in monkey cells, tissues and body fluids in immunofluorescence and immunoenzyme methods. for the production of immunoconjugates with a selected marker. to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier. as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e. g. ELISA). Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. Sometimes the diagnosis can be based on directly on laboratory findings. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:25 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:50 and 1:500.

Lyophilized from PBS, pH 7.2, No preservatives added

Reconstitute with distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.