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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Complement C2 Antibody IHC‑plus™ LS‑B15582
Note: This antibody replaces LS-C496896
Complement C2 antibody LS-B15582 is an unconjugated rabbit polyclonal antibody to Complement C2 from human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B15582-50
50 µl
$425
Description
Complement C2 antibody LS-B15582 is an unconjugated rabbit polyclonal antibody to Complement C2 from human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Complement C2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Complement C2 antibody was raised against recombinant fusion protein containing a sequence corresponding to amino acids 453-752 of human C2 (NP_000054.2). LDVSKLTDTICGVGNMSANASDQERTPWHVTIKPKSQETCRGALISDQWVLTAAHCFRDGNDHSLWRVNVGDPKSQWGKEFLIEKAVISPGFDVFAKKNQGILEFYGDDIALLKLAQKVKMSTHARPICLPCTMEANLALRRPQGSTCRDHENELLNKQSVPAHFVALNGSKLNINLKMGVEWTSCAEVVSQEKTMFPNLTDVREVVTDQFLCSGTQEDESPCKGESGGAVFLERRFRFFQVGLVSWGLYNPCLGSADKNSRKRAPRSKVPPPRDFHINLFRMQPWLRQHLGDVLNFLPL
Usage
The predicted MW is 60kDa/69kDa/83kDa, while the observed MW by Western blot was 70kDa/110kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About Complement C2
P06681 NM_000063 NP_000054.2

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Images

Immunohistochemistry - Paraffin

Human Small Intestine, Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine, Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of extracts of various cell lines, using C2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
Western blot analysis of extracts of various cell lines, using C2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.

Immunohistochemistry - Paraffin

Human Small Intestine, Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine, Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of extracts of various cell lines, using C2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
Western blot analysis of extracts of various cell lines, using C2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.

Immunohistochemistry - Paraffin

Human Small Intestine, Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine, Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of extracts of various cell lines, using C2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
Western blot analysis of extracts of various cell lines, using C2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.

Immunohistochemistry - Paraffin

Human Small Intestine, Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine, Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of extracts of various cell lines, using C2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
Western blot analysis of extracts of various cell lines, using C2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.

Requested From: United States
Date Requested: 11/21/2018
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