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Catalog Number Size Price
LS-C744067-0.1 0.1 ml $510 
LS-C744067-0.5 0.5 ml (1 mg/ml) $869 
Collagen IV Antibody - Collagen Type IV on mouse skin DAB, hematoxylin

Polyclonal Rabbit anti‑Mouse Collagen IV Antibody (IHC, IF) LS‑C744067

Polyclonal Rabbit anti‑Mouse Collagen IV Antibody (IHC, IF) LS‑C744067

Antibody:
Collagen IV Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC-P, IF, ELISA, RIA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$510
LS-C744067-0.1
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Collagen IV Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC-P, IF, ELISA, RIA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Collagen IV antibody LS-C744067 is an unconjugated rabbit polyclonal antibody to mouse Collagen IV. Validated for ELISA, IF, IHC and RIA.
Target
Mouse Collagen IV
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
Purified collagen type IV from murine tumour tissue
Applications
  • IHC - Paraffin (1:1000)
  • Immunofluorescence (1:80)
  • ELISA (1:200)
  • Radioimmunoassay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Murine collagen type IV 100%; murine collagen type I, II and III <0.1%; human collagen type IV and V <0.1%; murine fibronectin <0.1%; murine laminin <0.1% in RIA at 1:5000 dilution.
Presentation
PBS
Reconstitution
0.5 ml Reconstitute in sterile distilled water.
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 1/23/2022