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Catalog Number Size Price
LS-C744077-0.5 0.5 ml (1 mg/ml) $869 
Antibody

Polyclonal Rabbit anti‑Chicken COL9A3 / Collagen IX Antibody (IHC, IF) LS‑C744077

Polyclonal Rabbit anti‑Chicken COL9A3 / Collagen IX Antibody (IHC, IF) LS‑C744077

Antibody:
COL9A3 / Collagen IX Rabbit anti-Chicken Polyclonal Antibody
Application:
IHC-P, IF, ELISA, RIA
Reactivity:
Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$869
LS-C744077-0.5
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
COL9A3 / Collagen IX Rabbit anti-Chicken Polyclonal Antibody
Application:
IHC-P, IF, ELISA, RIA
Reactivity:
Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
Collagen IX antibody LS-C744077 is an unconjugated rabbit polyclonal antibody to chicken Collagen IX (COL9A3). Validated for ELISA, IF, IHC and RIA.
Target
Chicken COL9A3 / Collagen IX
Synonyms
COL9A3 | Collagen alpha 3(x) | Collagen alpha-3(IX) chain | Collagen type IX proteoglycan | EDM3 | DJ885L7.4.1 | IDD | MED | Collagen, type IX, alpha 3
Host
Rabbit
Reactivity
Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
Purified collagen type IX from chicken breast bone
Applications
  • IHC - Paraffin (1:200 - 1:600)
  • Immunofluorescence (1:40)
  • ELISA
  • Radioimmunoassay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Collagen type IX is found as non-fibrillary structure protein (68-115 kDa) cosisting of alpha-1(IX), alpha-2(IX) and alpha-3(IX) collagen in cartilage and vitreous body. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Chicken collagen type IX 100%, chicken collagen type I, II, III and V negative in ELISA at 1:100 dilution.
Presentation
PBS
Reconstitution
0.5 ml Reconstitute in sterile distilled water.
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About COL9A3 / Collagen IX
NM_001853

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 1/18/2022