Collagen VIII antibody LS-C135494 is an unconjugated rabbit polyclonal antibody to Collagen VIII (COL8A2) from human and zebrafish. Validated for WB.
Human COL8A2 / Collagen VIII
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
Synthetic peptide located between aa285-334 of human COL8A2 (P25067, NP_005193). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Zebrafish (100%); Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (92%); Turkey, Zebra finch, Chicken, Medaka, Drosophila (84%).
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
About COL8A2 / Collagen VIII