Mammal Collagen IV

Rabbit

Mammal, Human, Bovine (tested or 100% immunogen sequence identity)

IgG Polyclonal

Horseradish Peroxidase

Affinity chromatography

Unmodified

Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta.

Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

• IHC (1:1000 - 1:5000)
• Western blot (1:2000 - 1:10000)
• Immunoprecipitation (1:100)
• ELISA (1:20000 - 1:100000)

Applications should be user optimized.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.01 M Sodium Phosphate, pH 7.2, 0.25 M NaCl, 0.01% Thimerosal

Reconstitute in 50µL of deionized water.

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.